Katzenellenbogen B S
Endocrinology. 1975 Feb;96(2):289-97. doi: 10.1210/endo-96-2-289.
Analysis of newly labeled soluble proteins in uteri of mature rats throughout the estrous cycle indicates that the relative rate of synthesis of the uterine estrogen-induced protein, IP, is high at proestrus, when endogenous estrogen secretion is maximal; it is not synthesized at detectable levels at estrus and metestrus; and some minimal synthesis is seen in diestrus uteri. Injection of exogenous estrogen results in some increase in the IP synthesis rate at proestrus; slight induction of IP synthesis at estrus; and maximal induction (as great as that induced in the mature, ovariectomized uterus by estrogen) at metestrus and diestrus. Studies in the immature (day 21-24) rat, aimed at determining the possible causes of the uterine recalcitrance to exogenous estrogen seen at estrus, indicate that one can reproduce in the immature uterus a period of feeble responsiveness to a second injection of estrogen after exposure to a first, high dose of estrogen. After a single injection of estrogen, there is a lag between the gradual return of full IP inducibility (requiring over 40 hours to reach the control level) and the return of nuclear-translocatable receptor (at control level by 24 hr). This suggests that in addition to the presence of nuclear-translocatable receptor, the response to a second injection of estrogen is dependent upon other factors whose replenishment and/or reactivation is slower than that of the receptor.
对处于动情周期的成熟大鼠子宫中新标记的可溶性蛋白质进行分析表明，子宫雌激素诱导蛋白（IP）的相对合成速率在动情前期较高，此时内源性雌激素分泌达到最大值；在动情期和动情后期未检测到其合成；在间情期子宫中可见少量合成。注射外源性雌激素会使动情前期的IP合成速率有所增加；动情期会轻微诱导IP合成；在动情后期和间情期则会出现最大诱导（与雌激素诱导成熟、去卵巢子宫中的情况一样大）。对未成熟（第21 - 24天）大鼠的研究旨在确定动情期子宫对外源性雌激素产生抵抗的可能原因，结果表明，在未成熟子宫中，在首次暴露于高剂量雌激素后，再次注射雌激素时可以重现一段对其反应较弱的时期。单次注射雌激素后，完全IP诱导能力的逐渐恢复（需要超过40小时才能达到对照水平）与核可转运受体的恢复（24小时达到对照水平）之间存在滞后。这表明，除了存在核可转运受体外，对第二次注射雌激素的反应还取决于其他因素，这些因素的补充和/或重新激活比受体的要慢。
