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通过实时聚合酶链反应对生物样品中的克劳氏芽孢杆菌进行定量分析。

Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction.

作者信息

Perotti Mario, Mancini Nicasio, Cavallero Annalisa, Carletti Silvia, Canducci Filippo, Burioni Roberto, Clementi Massimo

机构信息

Università Vita-Salute San Raffaele and Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milan, Italy.

出版信息

J Microbiol Methods. 2006 Jun;65(3):632-6. doi: 10.1016/j.mimet.2005.10.010. Epub 2005 Nov 28.

DOI:10.1016/j.mimet.2005.10.010
PMID:16318892
Abstract

A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo.

摘要

开发并优化了一种针对克劳氏芽孢杆菌DNA高度特异性erm34序列的实时PCR检测方法。定量检测显示样品的灵敏度水平为10(2) CFU/微升。该方法可能是监测克劳氏芽孢杆菌作为体内益生菌作用的有用工具。

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