[中药隔药灸治疗大鼠溃疡性结肠炎差异表达基因的研究]
[Study on differentially expressed genes of ulcerative colitis in the rat treated by herbs-partitioned moxibustion].
作者信息
Wu Huan-Gan, Liu Hui-Rong, Zhao Chen, Zhang Wei, Wu Xue-Fei, Zhou Shuang, Shi Yin, Liu Min
机构信息
Shanghai Research Institute of Acupuncture and Meridians, Shanghai 200030, China.
出版信息
Zhongguo Zhen Jiu. 2005 May;25(5):359-65.
OBJECTIVE
To probe the mechanism of herbs-partitioned moxibustion for treatment of ulcerative colitis.
METHODS
The rats with ulcerative colitis were randomly divided into 3 groups, normal control group, model group, and herbs-partitioned moxibustion group. The rats in the herbs-partitioned moxibustion group were treated by herbs-partitioned moxibustion at bilateral Tianshu (ST 25). BiostarR-40s gene chip was applied to detect the differentially expressed gene of their colonic tissues and fluorescence quantitative polymerase chain reaction was employed to confirm the results of the microarray analysis with interleukin-1 beta messenger RNA (IL-1beta mRNA) expression.
RESULTS
Of 174 differential expression genes identified, 28 genes (including 7 known genes) elevated in rats of ulcerative colitis were down-regulated and 146 genes (including 42 known genes) reduced in the rat of ulcerative colitis were up-regulated after herbs-partitioned moxibustion treatment.
CONCLUSION
Many abnormally expressed genes are involved in occurrence of ulcerative colitis and herbs-partitioned moxibustion can regulate expression of IL-1beta and other genes to exert therapeutic effect.
目的
探讨隔药饼灸治疗溃疡性结肠炎的作用机制。
方法
将溃疡性结肠炎大鼠随机分为3组，即正常对照组、模型组、隔药饼灸组。对隔药饼灸组大鼠双侧天枢穴（ST25）进行隔药饼灸治疗。应用BiostarR-40s基因芯片检测其结肠组织差异表达基因，采用荧光定量聚合酶链反应验证芯片分析结果中白细胞介素-1β信使核糖核酸（IL-1βmRNA）表达情况。
结果
经隔药饼灸治疗后，溃疡性结肠炎大鼠中174个差异表达基因，其中28个（含7个已知基因）上调基因下调，146个（含42个已知基因）下调基因上调。
结论
溃疡性结肠炎的发生涉及多个异常表达基因，隔药饼灸可调节IL-1β等基因表达发挥治疗作用。
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