On-bead fluorescence assay for serine/threonine kinases.
作者信息
Akita Shoji, Umezawa Naoki, Higuchi Tsunehiko
机构信息
Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Nagoya, Japan.
出版信息
Org Lett. 2005 Dec 8;7(25):5565-8. doi: 10.1021/ol052125k.
PMID:16320992
Abstract
[chemical reaction: see text]. A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.
摘要
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