Barcs I, Jánosi L
B. Johan National Institute of Hygiene, Budapest, Hungary.
Acta Microbiol Hung. 1992;39(1):85-92.
Two 1.7 Md plasmids of Staphylococcus epidermidis and three ones of Staphylococcus simulans determining inducible macrolide-lincosamide resistance are identical as judged by restriction endonuclease fingerprinting. These plasmids designated pEI2101, pEI9105, pE1107, pEI1108 and pEI6104, respectively, belong to the incompatibility group 12. Dot-blot hybridization by photobiotin-labelled gene probe developed from S. aureus erythromycin ribosomal methylase gene showed cross hybridization between methylase-coding reference plasmids and the tested ones. The examined plasmids proved to be no transmissible in mating experiments into S. aureus recipients.
通过限制性内切酶指纹图谱判断，表皮葡萄球菌的两个1.7Md质粒和模仿葡萄球菌的三个决定诱导型大环内酯 - 林可酰胺耐药性的质粒是相同的。这些分别命名为pEI2101、pEI9105、pE1107、pEI1108和pEI6104的质粒属于不相容群12。用从金黄色葡萄球菌红霉素核糖体甲基化酶基因开发的光生物素标记基因探针进行斑点杂交，结果显示甲基化酶编码参考质粒与测试质粒之间存在交叉杂交。在交配实验中，所检测的质粒被证明不能转移到金黄色葡萄球菌受体中。
