Vogel T, Singer M
J Biol Chem. 1975 Jan 25;250(2):796-8.
The interaction of supercoiled, circular SV40 DNA with calf thymus histone fractions has been studied. Five- to ten-fold less f1 histone is required to complex a given amount of DNA compared to the other histones. When the supercoiled DNA is converted to either the relaxed circular form, or full length linear molecules, or gragmented linear or denatured stands, the efficiency of complex formation with f1 histone markedly decreases. We conclude that f1 histone has a special ability to interact with supercoiled DNA. This conclusion is supported by the fact that supercoiled circular Col E1 DNA interacts with f1 as efficiently as does SV40 DNA.
已对超螺旋环状SV40 DNA与小牛胸腺组蛋白组分的相互作用进行了研究。与其他组蛋白相比，使一定量的DNA形成复合物所需的f1组蛋白要少5至10倍。当超螺旋DNA转变为松弛环状形式、全长线性分子、片段化线性分子或变性单链时，与f1组蛋白形成复合物的效率会显著降低。我们得出结论，f1组蛋白具有与超螺旋DNA相互作用的特殊能力。超螺旋环状Col E1 DNA与f1的相互作用效率与SV40 DNA相同，这一事实支持了该结论。
