Charest Joseph L, Eliason Marcus T, García Andrés J, King William P
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 771 Ferst Drive NW, Atlanta, GA 30332-0405, USA.
Biomaterials. 2006 Apr;27(11):2487-94. doi: 10.1016/j.biomaterials.2005.11.022. Epub 2005 Dec 2.
This paper presents a technique to independently form mechanical topography and surface chemical patterns on polymer cell substrates, and studies the response of osteoblast cells to these surface patterns. The patterns were formed in two separate steps: hot embossing imprint lithography formed the mechanical topography and microcontact printing created the chemical pattern. The resulting substrate had surface features consisting of embossed grooves 4 microm deep and 8 microm wide spaced by 16 microm wide mesas and microcontact printed adhesive lanes 10 microm wide with spacings that ranged from 10 to 100 microm. When presented with either mechanical topography or chemical patterns alone, the cells significantly aligned to the pattern presented. When presented with mechanical topography overlaid with an orthogonal chemical pattern, the cells aligned to the mechanical topography. As the chemical pattern spacing was increased, osteoblasts remained aligned to the mechanical topography. Unlike traditional microfabrication approaches based on photolithography and wet chemistry, the patterning technique presented is compatible with a large number of biomaterials, could form patterns with features much smaller than 1 microm, and is highly scalable to large substrates.
本文介绍了一种在聚合物细胞基质上独立形成机械形貌和表面化学图案的技术，并研究了成骨细胞对这些表面图案的反应。图案通过两个独立步骤形成：热压印光刻形成机械形貌，微接触印刷产生化学图案。所得基质的表面特征包括4微米深、8微米宽的压花凹槽，由16微米宽的台面隔开，以及10微米宽的微接触印刷粘合剂通道，间距范围为10至100微米。当单独呈现机械形貌或化学图案时，细胞会显著与所呈现的图案对齐。当呈现叠加有正交化学图案的机械形貌时，细胞会与机械形貌对齐。随着化学图案间距的增加，成骨细胞仍与机械形貌对齐。与基于光刻和湿化学的传统微加工方法不同，本文介绍的图案化技术与大量生物材料兼容，可以形成特征尺寸远小于1微米的图案，并且高度可扩展到大尺寸基板。
