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壳聚糖/聚吡咯涂覆的聚（L-乳酸）/聚己内酯定向纤维膜的制备及其对神经细胞相容性和神经突生长的增强作用。

Fabrication of Chitosan/Polypyrrole-coated poly(L-lactic acid)/Polycaprolactone aligned fibre films for enhancement of neural cell compatibility and neurite growth.

机构信息

College of Materials Science and Engineering, Sichuan University, Chengdu, China.

出版信息

Cell Prolif. 2019 May;52(3):e12588. doi: 10.1111/cpr.12588. Epub 2019 Apr 11.

DOI:10.1111/cpr.12588
PMID:30972893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6536449/
Abstract

OBJECTIVE

Chitosan (CS) and polycaprolactone (PCL) were added into a nerve scaffold of poly(L-lactide acid) (PLLA)/polypyrrole (PPy)-based fibre films to solve the unmatch with the nerve strength and the aseptic inflammation from PLLA.

METHODS

Poly (L-lactide acid)-polycaprolactone (PLLA/PCL) fibre films coated with chitosan (CS) and polypyrrole (PPy) were prepared by electrospinning of aligned PLLA/PCL fibres, electrochemical deposition of PPy nanoparticles and in situ doping of CS in PPy. PC12 cells were electrically stimulated with 100 mV for 2 hours every day via CS/PPy-PLLA/PCL fibre film to promote the neurite growth.

RESULTS

The surface conductivity and tensile strength of CS/PPy-PLLA/PCL fibre films were 1.03 s/m and 13 MPa, respectively. CS content in fibre films was about 7.5 mg/cm , improving the pH value (reached to 5.1) of immersion solution of the fibre film at 16 days. Compared with PPy-PLLA/PCL fibre film, more and longer axons were grown out from PC12 cells cultured on CS/PPy-PLLA/PCL fibre film, indicating the positive effect of CS in fibre film on axon growth. The cell differentiation rate and neurite length on CS/PPy-PLLA/PCL fibre film reached to 38% and 75 μm, respectively. These results suggest the promotion of electrical stimulation on neurite growth and alignment.

CONCLUSIONS

A synergistic mechanism about the promotion of CS, electrical stimulation and aligned fibres on PC12 cells differentiation, axon outgrowth was proposed. These results indicated the potential application of CS/PPy-PLLA/PCL fibre film in the field of the nerve repair and regeneration.

摘要

目的

壳聚糖（CS）和聚己内酯（PCL）被添加到聚（L-丙交酯）（PLLA）/聚吡咯（PPy）基纤维膜的神经支架中，以解决与神经强度不匹配和 PLLA 的无菌炎症问题。

方法

通过 PLLA/PCL 纤维的定向电纺、PPy 纳米颗粒的电化学沉积和 PPy 中的 CS 原位掺杂，制备了涂有壳聚糖（CS）和聚吡咯（PPy）的聚（L-丙交酯）-聚己内酯（PLLA/PCL）纤维膜。通过 CS/PPy-PLLA/PCL 纤维膜每天对 PC12 细胞施加 100 mV 的电刺激 2 小时，以促进轴突生长。

结果

CS/PPy-PLLA/PCL 纤维膜的表面电导率和拉伸强度分别为 1.03 s/m 和 13 MPa。纤维膜中的 CS 含量约为 7.5 mg/cm，在第 16 天提高了纤维膜浸出液的 pH 值（达到 5.1）。与 PPy-PLLA/PCL 纤维膜相比，更多和更长的轴突从培养在 CS/PPy-PLLA/PCL 纤维膜上的 PC12 细胞中生长出来，表明纤维膜中 CS 对轴突生长有积极作用。CS/PPy-PLLA/PCL 纤维膜上的细胞分化率和神经突长度分别达到 38%和 75 μm。这些结果表明电刺激对神经突生长和排列的促进作用。

结论

提出了 CS、电刺激和定向纤维对 PC12 细胞分化、轴突生长的协同作用机制。这些结果表明 CS/PPy-PLLA/PCL 纤维膜在神经修复和再生领域具有潜在的应用前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/a87f9a5e467d/CPR-52-e12588-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/b640cae6202c/CPR-52-e12588-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/d294148928b7/CPR-52-e12588-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/a8168d808c44/CPR-52-e12588-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/f6768d0c6821/CPR-52-e12588-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/ec0d36a2684d/CPR-52-e12588-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/a87f9a5e467d/CPR-52-e12588-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/b640cae6202c/CPR-52-e12588-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/d294148928b7/CPR-52-e12588-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/a8168d808c44/CPR-52-e12588-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/f6768d0c6821/CPR-52-e12588-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/ec0d36a2684d/CPR-52-e12588-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdbd/6536449/a87f9a5e467d/CPR-52-e12588-g006.jpg

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