Johnson Shawn, Lowenstine Linda, Gulland Frances, Jang Spencer, Imai Denise, Almy Frederic, Delong Robert, Gardner Ian
Veterinary Medical Teaching Hospital, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, One Shields Ave., University of California, Davis, 95616, USA.
Vet Microbiol. 2006 Apr 16;114(1-2):94-103. doi: 10.1016/j.vetmic.2005.11.045. Epub 2005 Dec 2.
To investigate the association between genital bacterial infection and urogenital carcinoma in California sea lions (Zalophus californianus), vaginal and preputial swabs for bacterial isolation were taken from 148 free-ranging and 51 stranded California sea lions including 16 animals with urogenital carcinoma. Cytological examination of vaginal or preputial smears showed a majority (65.5%, 57/87) of animals examined had mild or no inflammation. Aerobic bacteria were isolated from 116 (78.4%) wild sea lions and 100% of stranded animals. A total of 403 isolates were identified representing 51 unique bacterial species. The median number of isolates per animal increased with age in the wild group, but there was no difference in the number of isolates per animal between wild and stranded adults. The most common bacteria isolated from the wild sea lions were Psychrobacter phenylpyruvicus (39 isolates), non-hemolytic Streptococcus (35 isolates), Corynebacterium spp. (30 isolates), and Escherichia coli (20 isolates). More bacterial species were isolated from stranded animals than wild animals (33 versus 26) and there was significantly less growth of P. phenylpyruvicus, Corynebacterium spp., and Moraxella-like spp. in the stranded animals. Beta-hemolytic Streptococcus was the only bacterium significantly associated with urogenital carcinomas in California sea lions, but only in females.
为研究加利福尼亚海狮（Zalophus californianus）生殖道细菌感染与泌尿生殖系统癌之间的关联，从148只自由放养和51只搁浅的加利福尼亚海狮身上采集阴道和包皮拭子用于细菌分离，其中包括16只患有泌尿生殖系统癌的动物。对阴道或包皮涂片进行细胞学检查发现，大多数（65.5%，57/87）受检动物有轻度炎症或无炎症。从116只（78.4%）野生海狮和100%的搁浅动物中分离出需氧菌。共鉴定出403株分离菌，代表51种独特的细菌物种。野生组中，每只动物分离菌的中位数随年龄增加而增多，但野生成年海狮和搁浅成年海狮每只动物的分离菌数量没有差异。从野生海狮中分离出的最常见细菌是苯丙酮酸嗜冷杆菌（39株）、非溶血性链球菌（35株）、棒状杆菌属（30株）和大肠杆菌（20株）。从搁浅动物中分离出的细菌种类比野生海狮多（33种对26种），并且搁浅动物中苯丙酮酸嗜冷杆菌、棒状杆菌属和莫拉克斯氏菌属样菌的生长明显较少。β-溶血性链球菌是唯一与加利福尼亚海狮泌尿生殖系统癌显著相关的细菌，但仅在雌性海狮中相关。
