Buckles E L, Lowenstine L J, Funke C, Vittore R K, Wong H-N, St Leger J A, Greig Denise J, Duerr R S, Gulland F M D, Stott J L
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, One Shields Drive, University of California, Davis, CA 95616, USA.
J Comp Pathol. 2006 Nov;135(4):183-9. doi: 10.1016/j.jcpa.2006.06.007. Epub 2006 Oct 10.
The purpose of this study was to determine if Otarine Herpesvirus-1 (OtHV-1) is associated with the presence of urogenital carcinomas in California sea lions. Polymerase chain reaction (PCR) analysis with primers specific for OtHV-1 was used to compare the prevalence of OtHV-1 infection in 15 sea lions affected by urogenital carcinoma with that of age-matched and juvenile tumour-free animals, and animals with tumours of non-urogenital origin. The herpesvirus was more prevalent (100%) and more widespread in the 15 animals with urogenital carcinoma than in 25 control animals, and was most often found in the urogenital tissue (vagina and prostate) and in the draining lymph nodes. Moreover, OtHV-1 DNA was not found in any juvenile animal, or in the neoplastic tissues of animals with non-urogenital tumours. Papillomavirus-specific PCR analysis of urogenital carcinoma tissues detected papillomavirus sequences in only one carcinomatous tissue. Further studies are needed to determine if OtHV-1 contributes to oncogenesis in the California sea lion; these data show, however, that OtHV-1 is associated with urogenital carcinomas, is preferentially present in urogenital tissues, and may be sexually transmitted. Papillomaviruses, which are known to contribute to urogenital tumours in other species, did not appear to be associated with the sea lion carcinomas.
本研究的目的是确定海狗疱疹病毒-1（OtHV-1）是否与加利福尼亚海狮泌尿生殖系统癌的发生有关。使用针对OtHV-1的特异性引物进行聚合酶链反应（PCR）分析，以比较15只患有泌尿生殖系统癌的海狮、年龄匹配且无肿瘤的幼年海狮以及患有非泌尿生殖系统来源肿瘤的动物中OtHV-1感染的患病率。与25只对照动物相比，疱疹病毒在15只患有泌尿生殖系统癌的动物中更为普遍（100%）且分布更广，并且最常出现在泌尿生殖组织（阴道和前列腺）以及引流淋巴结中。此外，在任何幼年动物或患有非泌尿生殖系统肿瘤的动物的肿瘤组织中均未发现OtHV-1 DNA。对泌尿生殖系统癌组织进行乳头瘤病毒特异性PCR分析，仅在一个癌组织中检测到乳头瘤病毒序列。需要进一步研究以确定OtHV-1是否在加利福尼亚海狮的肿瘤发生中起作用；然而，这些数据表明，OtHV-1与泌尿生殖系统癌有关，优先存在于泌尿生殖组织中，并且可能通过性传播。已知在其他物种中可导致泌尿生殖系统肿瘤的乳头瘤病毒似乎与海狮的癌症无关。
