Wu Chi-Ming, Lee Yun-Shien, Wang Tzu-Hao, Lee Li-Yu, Kong Wei-Hsiang, Chen En-Shih, Wei Min-Li, Liang Ying, Hwang Tsann-Long
Department of Biotechnology, Vanung University, Tao-Yuan, Taiwan.
Oncol Rep. 2006 Jan;15(1):57-64.
To compare the gene expression profiling between intestinal-type gastric cancer (IGC) and diffuse-type gastric cancer (DGC), cDNA microarray containing 7334 gene elements was performed on 12 paired IGC specimens/its' normal epithelial tissue and 11 paired DGC specimens/its' normal epithelial tissue. Twenty-seven genes were co-overexpressed in IGC and DGC. These overexpressed genes were related to transcription and translation, DNA replications and mitosis, calcium binding, apoptosis and mitochondria protein. Twelve genes were co-underexpressed in IGC and DGC. These underexpressed genes were associated with cell adhesion and migration, organelle movement and intracellular transport, and matrix metalloproteinase. A clustering dendrogram of IGC and DGC with 27 genes significantly differed between IGC and DGC. Nineteen genes were more overexpressed in DGC than in IGC, including annexin A1 (ANXA1), chemokine ligand 7 (CCL7), and chemokine ligand 8 (CCL8). Eight genes were more overexpressed in IGC than in DGC, including claudin 4 (CLDN4). The results of quantitative real-time PCR and immunohistochemical staining confirmed the microarray finding. The gene expression profiling between IGC and DGC suggested that they might have unique genetic pathways which share some of the same and some different genetic alterations.
为比较肠型胃癌（IGC）和弥漫型胃癌（DGC）之间的基因表达谱，我们对12对IGC标本及其正常上皮组织以及11对DGC标本及其正常上皮组织进行了包含7334个基因元件的cDNA微阵列分析。有27个基因在IGC和DGC中共同过表达。这些过表达的基因与转录和翻译、DNA复制和有丝分裂、钙结合、凋亡以及线粒体蛋白有关。有12个基因在IGC和DGC中共同低表达。这些低表达的基因与细胞黏附与迁移、细胞器运动和细胞内运输以及基质金属蛋白酶有关。IGC和DGC基于27个基因的聚类树状图在IGC和DGC之间存在显著差异。有19个基因在DGC中的过表达高于IGC，包括膜联蛋白A1（ANXA1）、趋化因子配体7（CCL7）和趋化因子配体8（CCL8）。有8个基因在IGC中的过表达高于DGC，包括紧密连接蛋白4（CLDN4）。定量实时PCR和免疫组织化学染色结果证实了微阵列分析的发现。IGC和DGC之间的基因表达谱表明它们可能具有独特的遗传途径，这些途径既有一些相同的遗传改变，也有一些不同的遗传改变。
