Li Ke, Yao Ping, Zhou Shao-Liang, Song Fang-Fang
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2005 Sep;34(5):559-62.
This study was designed to investigate whether EGB protect against ethanol-induced oxidative injury in rat testes.
The rats were pretreated with EGB (4.8,9.6 mg/100g bw per day) before 30% ethanol administration (2.37g/kg bw by gastric incubation). Ninety days later, the rats were killed in order to measure the activities of Superoxide Dismutase (SOD), Glutathione Reductase (GST), Glutathione Peroxidase (GSH-Px), Catalase (CAT) and the contents of Glutathione (GSH), Reactive Oxygen Species (ROS), Malondialdehyde (MDA)in rat testes. The microsomal fractions were obtained by the method of differential centrifugation, and HO-1 (Heme Oxygenase-1) activity in testicular microsomal was measured. To examine the expression of HO-1 mRNA by reverse transcription polymerase chain reaction (RT-PCR).
Compared with ethanol group, ROS, and MDA contents significantly decreased in EGB group testes, respectively (P < 0.05). GST, GSH-Px, CAT, SOD activities, and GSH content in EGB group testes elevated markedly (P <0.05). EGB could enhance HO-1 mRNA expression remarkably.
It is suggested that EGB as a preventive antioxidant can protect against ethanol-induced testicular injury, which may be associated with HO-1 activity enhancement, free radicals elimination, lipid per-oxidation reaction inhibition.
本研究旨在探讨银杏叶提取物（EGB）是否能预防乙醇诱导的大鼠睾丸氧化损伤。
在给予30%乙醇（通过胃内灌胃，剂量为2.37g/kg体重）之前，用EGB（4.8、9.6mg/100g体重/天）对大鼠进行预处理。90天后，处死大鼠以测量大鼠睾丸中超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GST）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）的活性以及谷胱甘肽（GSH）、活性氧（ROS）、丙二醛（MDA）的含量。采用差速离心法获得微粒体组分，并测定睾丸微粒体中血红素加氧酶-1（HO-1）的活性。通过逆转录聚合酶链反应（RT-PCR）检测HO-1 mRNA的表达。
与乙醇组相比，EGB组睾丸中ROS和MDA含量显著降低（P<0.05）。EGB组睾丸中GST、GSH-Px、CAT、SOD活性以及GSH含量显著升高（P<0.05）。EGB可显著增强HO-1 mRNA的表达。
提示EGB作为一种预防性抗氧化剂可预防乙醇诱导的睾丸损伤，这可能与增强HO-1活性及清除自由基、抑制脂质过氧化反应有关。
