Lee I-M, Zhao Y, Bottner K D
USDA-ARS Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA.
Mol Cell Probes. 2006 Apr;20(2):87-91. doi: 10.1016/j.mcp.2005.10.001. Epub 2005 Dec 5.
Aster yellows (AY) group (16SrI) phytoplasmas are associated with more than 100 economically important diseases worldwide and represent the most diverse and widespread phytoplasma group. Phylogenetic analysis of secY gene sequences resolved 10 genetically distinct lineages. The 10 lineages coincide with those delineated by phylogenetic analysis based on ribosomal protein (rp) gene sequences. However, greater genetic variability among the 10 lineages was revealed based on secY gene sequences. The distinct phylogenetic lineages can be readily identified through restriction fragment length polymorphism (RFLP) analysis of secY gene sequences. Ten subgroups were differentiated among the AY group phytoplasmas based on RFLP analysis of secY gene sequences. Phylogenetic analysis based on secY gene sequences in this study, and previous studies on the 16S rRNA gene, tuf gene, and rp gene sequences reinforced the notion that most subgroups identified by RFLP analysis of secY and rp gene sequences represent distinct phylogenetic lineages.
翠菊黄化（AY）组（16SrI）植原体与全球100多种具有经济重要性的病害相关，是最多样化且分布最广的植原体组。对secY基因序列进行系统发育分析确定了10个遗传上不同的谱系。这10个谱系与基于核糖体蛋白（rp）基因序列的系统发育分析所划分的谱系一致。然而，基于secY基因序列揭示了这10个谱系之间更大的遗传变异性。通过对secY基因序列进行限制性片段长度多态性（RFLP）分析，可以很容易地识别出不同的系统发育谱系。基于secY基因序列的RFLP分析，在AY组植原体中区分出了10个亚组。本研究基于secY基因序列以及先前对16S rRNA基因、tuf基因和rp基因序列的研究进行的系统发育分析，强化了这样一种观念，即通过对secY和rp基因序列进行RFLP分析所鉴定的大多数亚组代表了不同的系统发育谱系。
