Schumm D E, Webb T E
J Natl Cancer Inst. 1975 Jan;54(1):123-8. doi: 10.1093/jnci/54.1.123.
Putative regulatory macromolecules, which may be useful in diagnosis or tumor detection, were identified in the peripheral blood plasma of tumor-bearing animals. We monitored the components by measuring their ability to stimulate messenger RNA (mRNA) release from isolated nuclei in a cell-free system of rat liver nuclei in fortified homologous cytosol. This in vitro test system exhibited near-normal in vivo nuclear RNA restriction. When added to the assay at a protein concentration of 3.0 mg/ml, dialyzed plasma from rats or mice with chemically induced transplantable or primary tumors stimulated mRNA release from 87% to more than 300% over control plasma from normal rats. Plasma from partially hepatectomized rats stimulated only 26% over control plasma. The test system derived from rat liver seemed to permit the monitoring of plasma from other species. Available evidence, particularly relating to tumor-host-interaction, suggests, but does not prove, that regulatory components are released from the tumor cells to the circulation.
在荷瘤动物的外周血血浆中鉴定出了可能有助于诊断或肿瘤检测的假定调节大分子。我们通过测量它们在强化同源胞质溶胶中的大鼠肝细胞核无细胞系统中刺激信使核糖核酸（mRNA）从分离的细胞核中释放的能力来监测这些成分。该体外测试系统表现出接近正常的体内核RNA限制。当以3.0 mg/ml的蛋白质浓度添加到测定中时，来自化学诱导的可移植或原发性肿瘤的大鼠或小鼠的透析血浆刺激mRNA释放的程度比正常大鼠的对照血浆高出87%至300%以上。部分肝切除大鼠的血浆刺激程度仅比对照血浆高26%。源自大鼠肝脏的测试系统似乎允许监测其他物种的血浆。现有证据，特别是与肿瘤-宿主相互作用相关的证据，表明但未证明调节成分从肿瘤细胞释放到循环中。
