Lee June-Der, Tsai Li-Yu, Chen Chun-Hsiang, Wang Jiun-Jye, Hsiao Jen-Kuei, Yen Chuan-Min
Department of Parasitology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan.
Acta Trop. 2006 Feb;97(2):204-11. doi: 10.1016/j.actatropica.2005.11.003. Epub 2005 Dec 5.
Several indices were used to assess whether blood-brain barrier (BBB) damage occurs in neurological disorders. Dysfunction of the BBB was surmised to be involved in the pathological changes of eosinophilic meningitis caused by the infection of Angiostrongylus cantonensis. The mean concentration of protein and albumin in the cerebrospinal fluid (CSF) of infected mice gradually increased from days 0 to 18 after infection and then rapidly increased 21 days after infection. The concentrations of protein and albumin in the CSF of infected mice 15 days after infection were all significantly higher than those in uninfected mice (all P-values at least <0.05). Parallel with the increase in protein and albumin in the CSF, infected mice showed a gradual increase in their CSF/serum protein and albumin ratios. The increase became significant at days 21 and 18 after infection, respectively (P<0.01 and P<0.05, respectively). The higher the worm counts in the brain, the higher the CSF/serum albumin ratio was observed in infected mice at day 21 after infection (P<0.001). In addition, the ratios of the CSF/serum albumin were positively correlated with the worm counts in the brain (P<0.001). The total leukocyte and eosinophil counts were also positively correlated with ratios of CSF/serum albumin (P<0.01). The amount of Evans blue in the brain of mice 21 days after infection from peripheral blood via BBB became significantly increased than those in uninfected mice (P<0.001). Thus, the evidence of high concentrations of protein and albumin, high leukocyte counts in CSF, high ratio of CSF/serum protein and albumin, and high permeability of BBB show that dysfunction of the BBB occurred in mice infected with A. cantonensis.
使用了几个指标来评估神经疾病中血脑屏障（BBB）是否受损。推测BBB功能障碍与广州管圆线虫感染引起的嗜酸性粒细胞性脑膜炎的病理变化有关。感染小鼠脑脊液（CSF）中的蛋白质和白蛋白平均浓度在感染后0至18天逐渐升高，然后在感染后21天迅速升高。感染后15天，感染小鼠脑脊液中的蛋白质和白蛋白浓度均显著高于未感染小鼠（所有P值均至少<0.05）。随着脑脊液中蛋白质和白蛋白的增加，感染小鼠的脑脊液/血清蛋白质和白蛋白比值逐渐升高。分别在感染后21天和18天，这种升高变得显著（分别为P<0.01和P<0.05）。感染后21天，感染小鼠脑内虫数越高，脑脊液/血清白蛋白比值越高（P<0.001）。此外，脑脊液/血清白蛋白比值与脑内虫数呈正相关（P<0.001）。总白细胞和嗜酸性粒细胞计数也与脑脊液/血清白蛋白比值呈正相关（P<0.01）。感染后21天，经BBB从外周血进入小鼠脑内的伊文思蓝量比未感染小鼠显著增加（P<0.001）。因此，蛋白质和白蛋白浓度高、脑脊液中白细胞计数高、脑脊液/血清蛋白质和白蛋白比值高以及BBB通透性高的证据表明，感染广州管圆线虫的小鼠出现了BBB功能障碍。
