Buskila D, Weigl D, Shoenfeld Y
Department of Medicine B, Sheba Medical Center, Tel Hashomer, Israel.
Hum Antibodies Hybridomas. 1992 Apr;3(2):75-80.
The sera of 340 patients with monoclonal gammopathies were examined for the presence of anti-Ro/SS-A and anti-La/SS-B activity. An enzyme-linked immunosorbent assay (ELISA) technique was employed. Forty-six sera (13.5%) bound to Ro/SS-A, while 79 sera (23.2%) bound to La/SS-B. The anti-Ro/SS-A and anti-La/SS-B antibodies were found in sera of patients with IgG, IgM, and IgA gammopathies. Forty-two of the 46 sera (91.3%) with positive anti-Ro/SS-A activity were found to bind La/SS-B as well, while only 53.2% (42 out of 79) of the sera that had anti-La/SS-B activity also bound Ro/SS-A. The activity against Ro/SS-A and La/SS-B was further confirmed by immunoblotting with purified immunoglobulins. Antibodies to Ro/SS-A and La/SS-B are common in systemic lupus erythematosus, Sjögren's syndrome, and other rheumatic disorders. Yet none of the patients whose serum was found to contain high titres of anti-Ro/SS-A or anti-La/SS-B human monoclonal antibodies presented with symptoms related to such autoimmune diseases. Our results of a high incidence of anti-Ro/SS-A or anti-La/SS-B activity in the sera of patients with monoclonal gammopathies support previous reports of autoantibody properties characteristic of these immunoglobulins.
检测了340例单克隆丙种球蛋白病患者血清中抗Ro/SS-A和抗La/SS-B活性。采用酶联免疫吸附测定（ELISA）技术。46份血清（13.5%）与Ro/SS-A结合，而79份血清（23.2%）与La/SS-B结合。在IgG、IgM和IgA丙种球蛋白病患者的血清中发现了抗Ro/SS-A和抗La/SS-B抗体。46份抗Ro/SS-A活性阳性的血清中有42份（91.3%）也被发现与La/SS-B结合，而在79份具有抗La/SS-B活性的血清中，只有53.2%（42份）也与Ro/SS-A结合。通过用纯化的免疫球蛋白进行免疫印迹进一步证实了针对Ro/SS-A和La/SS-B的活性。抗Ro/SS-A和抗La/SS-B抗体在系统性红斑狼疮、干燥综合征和其他风湿性疾病中很常见。然而，血清中被发现含有高滴度抗Ro/SS-A或抗La/SS-B人单克隆抗体的患者均未出现与这些自身免疫性疾病相关的症状。我们关于单克隆丙种球蛋白病患者血清中抗Ro/SS-A或抗La/SS-B活性高发的结果支持了先前关于这些免疫球蛋白自身抗体特性的报道。
