通过实时聚合酶链反应快速测定结核分枝杆菌临床分离株中的利福平耐药性
Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR.
作者信息
Kocagoz Tanil, Saribas Zeynep, Alp Alpaslan
机构信息
Department of Microbiology and Clinical Microbiology, Hacettepe University Faculty of Medicine, 06100 Sihhiye, Ankara, Turkey.
出版信息
J Clin Microbiol. 2005 Dec;43(12):6015-9. doi: 10.1128/JCM.43.12.6015-6019.2005.
Abstract
Real-time PCR was used to determine rifampin resistance in clinical isolates of Mycobacterium tuberculosis. Ninety-six rifampin-resistant isolates and 23 rifampin-susceptible isolates were included in the study. A 305-bp region covering the 81-bp "rifampin resistance-determining region" of rpoB was amplified. Two hybridization probe pairs that covered the most frequent mutation sites in rpoB, codon regions 526 to 531 and 513 to 516, were used. The results obtained by real-time PCR were compared to those obtained by the proportion method. For detection of rifampin resistance, the real-time PCR assay yielded a sensitivity of 92.7% and a specificity of 100%. Real-time PCR is a very rapid method, and it can be especially helpful for the reporting of resistant clinical isolates in a very short period of time.
摘要
采用实时聚合酶链反应（Real-time PCR）检测结核分枝杆菌临床分离株对利福平的耐药性。本研究纳入了96株利福平耐药分离株和23株利福平敏感分离株。扩增了覆盖rpoB基因81bp“利福平耐药决定区”的305bp区域。使用了两对覆盖rpoB基因最常见突变位点（密码子区域526至531和513至516）的杂交探针。将实时PCR获得的结果与比例法获得的结果进行比较。对于利福平耐药性的检测，实时PCR检测的灵敏度为92.7%，特异性为100%。实时PCR是一种非常快速的方法，对于在极短时间内报告耐药临床分离株特别有帮助。
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