结核分枝杆菌利福喷丁药敏试验的开发。
Development of rifapentine susceptibility tests for Mycobacterium tuberculosis.
作者信息
Heifets L, Sanchez T, Vanderkolk J, Pham V
机构信息
National Jewish Medical and Research Center and University of Colorado Health Sciences Center, Denver, Colorado 80206, USA.
出版信息
Antimicrob Agents Chemother. 1999 Jan;43(1):25-8. doi: 10.1128/AAC.43.1.25.
Abstract
Two methods for testing the susceptibility of Mycobacterium tuberculosis to rifapentine have been developed: the agar proportion method and the radiometric BACTEC technique. A critical concentration of 0.5 microg of rifapentine per ml is proposed for both methods since it provides a reliable means of distinguishing between susceptible and resistant M. tuberculosis isolates. It is recommended that two quality control M. tuberculosis strains be used at the introduction of these tests in a clinical laboratory: one that is pansusceptible (H37Rv) and one that is resistant to rifapentine. The resistant strain can be obtained from the American Type Culture Collection, where it is deposited under the number ATCC 700457.
摘要
已开发出两种检测结核分枝杆菌对利福喷汀敏感性的方法
琼脂比例法和放射性BACTEC技术。两种方法均建议利福喷汀的临界浓度为每毫升0.5微克，因为它提供了一种区分结核分枝杆菌敏感株和耐药株的可靠方法。建议临床实验室在引入这些检测时使用两种结核分枝杆菌质量控制菌株：一种是全敏感菌株（H37Rv），另一种是对利福喷汀耐药的菌株。耐药菌株可从美国典型培养物保藏中心获得，其保藏编号为ATCC 700457。
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