Moldes Cristina, Farinós Gema P, de Eugenio Laura I, García Pedro, García José L, Ortego Félix, Hernández-Crespo Pedro, Castañera Pedro, Prieto María A
Department of Molecular Microbiology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain.
UMR 163, CEA Cadarache, 13108 St. Paul lez Durance, Cedex, France.
Appl Microbiol Biotechnol. 2006 Aug;72(1):88-93. doi: 10.1007/s00253-005-0257-6. Epub 2005 Dec 7.
A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules through the polypeptide tag BioF. The new toxin, named Fk-Bt1, was shown to be active against Sesamia nonagrioides (Lepidoptera: Noctuidae). The dose-mortality responses of the new toxin granule formulation (PFk-Bt1) and purified Cry1Ab have been compared, demonstrating the effectiveness of PFk-Bt1 and suggesting a common mode of action.
基于聚羟基脂肪酸酯（PHA，生物塑料）颗粒的使用，已开发出一种以环保方式向环境中递送活性蛋白的新工具。为说明这种新方法，通过多肽标签BioF将一种衍生的Cry1Ab昆虫特异性毒素蛋白体内固定到PHA颗粒中。这种名为Fk-Bt1的新毒素对西班牙谷螟（鳞翅目：夜蛾科）具有活性。已比较了新毒素颗粒制剂（PFk-Bt1）和纯化的Cry1Ab的剂量-死亡率反应，证明了PFk-Bt1的有效性，并提示了一种共同的作用模式。
