Wu Shuai, Wang Xin-Sheng, Meng Fan-Hui
Department of Urology, Qingdao People's Hospital, Affiliated Hospital of Qingdao Medical University, Qingdao, Shandong 266003, China.
Zhonghua Nan Ke Xue. 2005 Nov;11(11):835-7.
To discuss the important role of actin polymerization in calcium ionophore A23187-induced human acrosome reaction and its mechanism.
Each spermatozoon specimen was divided into five groups, treated with A23187 3 micromol/L in Group A, Phalloidin 40 micromol/L and A23187 3 micromol/L in Group B, SLO 0.5 U/ml and A23187 3 micromol/L in Group C, SLO 0.5 U/ ml, Phalloidin 40 micromol/L and A23187 3 micromol/L in Group D, and nothing added in Grpup E. Then the percentage of the human acrosome reaction was assessed with Rhodamine-PSA (10 microg/ml).
The difference of the human spermatozoon acrosome reaction was significant (P < 0.01) among the 5 groups with or without SLO, Phalloidin and calcium ionophore A23187 but not between Groups A and B (P > 0.01).
Phalloidin does not work on the acrosome reaction of intact human spermatozoa, but in an SLO-permeabilized human spermatozoal model, it can obviously decrease the percentage of human spermatozoon acrosome reaction, which indicates that the polymerization of actin plays an important role in the course of human spermatozoon acrosome reaction, and mostly acts on the acrosome inside.
探讨肌动蛋白聚合在钙离子载体A23187诱导的人顶体反应中的重要作用及其机制。
将每份精子标本分为五组，A组用3 μmol/L A23187处理，B组用40 μmol/L鬼笔环肽和3 μmol/L A23187处理，C组用0.5 U/ml链球菌溶血素O（SLO）和3 μmol/L A23187处理，D组用0.5 U/ml SLO、40 μmol/L鬼笔环肽和3 μmol/L A23187处理，E组不添加任何物质。然后用罗丹明标记的前列腺特异性抗原（Rhodamine-PSA，10 μg/ml）评估人顶体反应的百分比。
在添加或不添加SLO、鬼笔环肽和钙离子载体A23187的五组之间，人精子顶体反应的差异有统计学意义（P < 0.01），但A组和B组之间差异无统计学意义（P > 0.01）。
鬼笔环肽对完整人精子的顶体反应无作用，但在SLO通透的人精子模型中，它能明显降低人精子顶体反应的百分比，这表明肌动蛋白聚合在人精子顶体反应过程中起重要作用，且主要作用于内部顶体。
