Miranda R C, Toran-Allerand C D
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Cereb Cortex. 1992 Jan-Feb;2(1):1-15. doi: 10.1093/cercor/2.1.1.
The distribution of estrogen receptor mRNA expression was studied in the developing rat cerebral cortex by in situ hybridization histochemistry. We used a specific, nonisotopically (digoxigenin) labeled, synthetic oligodeoxyribonucleotide complementary to a 48 base sequence in the region of the estrogen-binding domain of rat uterine estrogen receptor cDNA. During development, estrogen receptor mRNA was observed in all forebrain regions previously reported to bind estrogen, as determined by steroid autoradiography or nuclear binding assay. In the developing cerebral cortex, estrogen receptor mRNA was extensively expressed in the ventricular zone, primitive plexiform layer, and immature cortical plate at least as early as embryonic day 16. During the first 3 postnatal weeks, cortical mRNA expression was increasingly restricted to the upper third of the cerebral cortex and to the neurons of the cortical subplate (layer VIb/VII) and decreased to low levels by postnatal day 28. In the cerebral cortex, the spatial distribution of estrogen receptor mRNA expression overlapped that reported for the encoded protein. The extensive distribution of estrogen receptor mRNA throughout the late prenatal and early postnatal cerebral cortex points to an important role for estrogen in the differentiation and maturation of the cerebral cortex.
通过原位杂交组织化学方法研究了发育中大鼠大脑皮质中雌激素受体mRNA表达的分布情况。我们使用了一种特异性的、非同位素（地高辛）标记的合成寡脱氧核糖核苷酸，它与大鼠子宫雌激素受体cDNA雌激素结合域区域的48个碱基序列互补。在发育过程中，如通过类固醇放射自显影或核结合试验所确定的，在先前报道能结合雌激素的所有前脑区域均观察到了雌激素受体mRNA。在发育中的大脑皮质，至少早在胚胎第16天，雌激素受体mRNA就在脑室区、原始丛状层和未成熟皮质板中广泛表达。在出生后的前三周，皮质mRNA表达越来越局限于大脑皮质的上三分之一以及皮质下板（VIb/VII层）的神经元，到出生后第28天降至低水平。在大脑皮质中，雌激素受体mRNA表达的空间分布与所报道的编码蛋白的分布重叠。雌激素受体mRNA在整个产前后期和产后早期大脑皮质中的广泛分布表明雌激素在大脑皮质的分化和成熟中起重要作用。
