Mengod G, Pompeiano M, Martínez-Mir M I, Palacios J M
Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
Brain Res. 1990 Jul 30;524(1):139-43. doi: 10.1016/0006-8993(90)90502-3.
32P-labelled oligonucleotides complementary to rat 5-HT2 receptor mRNA were used as probes to study the distribution of cells in rat brain containing the mRNA coding for this receptor by in situ hybridization histochemistry. 5-HT2 receptor binding sites were visualized by autoradiography using [125I]DOI as ligand. Both distributions were comparable, demonstrating that 5-HT2 receptors are expressed by cells intrinsic to the neocortex (lamina Va), claustrum, olfactory bulb and several nuclei of the brainstem.
用与大鼠5-羟色胺2型（5-HT2）受体mRNA互补的32P标记寡核苷酸作为探针，通过原位杂交组织化学研究大鼠脑中含有该受体编码mRNA的细胞分布。使用[125I]DOI作为配体，通过放射自显影观察5-HT2受体结合位点。两种分布具有可比性，表明5-HT2受体由新皮层（第Va层）、屏状核、嗅球和脑干的几个核团中的固有细胞表达。
