Caglar G, Caglar S, Ergin O, Yarim M
Department of Horticulture, Faculty of Agriculture, Kahramanmaras Sutcu Imam University, Turkey.
J Environ Biol. 2005 Jul;26(3):479-85.
The objectives of this study were to examine the effect of TDZ and BAP on shoot proliferation and to develop a satisfactory rooting procedure for in vitro propagation of caper (Capparis spinosa L). TDZ was found to be more effective than BAP in shoot production. The highest shoot number per explant (45.7) was achieved on the media that contained 4.54 microM TDZ in the absence of IAA. TDZ at 4.54 microM alone or in combination with 0.57 microM IAA did not inhibit shoot elongation, although they produced the most shoots. Dipping the caper shoots in the solution of IBA at 24.6 microM for 10 minutes before planting on MS media, improved the rooting percentage up to 80.5% under a 16 hr photoperiod.
本研究的目的是检测噻苯隆（TDZ）和苄氨基嘌呤（BAP）对枝条增殖的影响，并为刺山柑（Capparis spinosa L）的离体繁殖开发一种令人满意的生根程序。结果发现，TDZ在芽苗生产方面比BAP更有效。在不含吲哚乙酸（IAA）且含有4.54微摩尔TDZ的培养基上，每个外植体的芽苗数最多（45.7个）。单独使用4.54微摩尔的TDZ或与0.57微摩尔IAA联合使用时，虽然产生的芽苗最多，但并未抑制芽苗伸长。将刺山柑枝条在种植到MS培养基之前，于24.6微摩尔的吲哚丁酸（IBA）溶液中浸泡10分钟，在16小时光周期下，生根率提高到了80.5%。
