Yu Ping, Liu Jin, Zhang Li, Li Shrng-Fu, Bu Hong, Li You-Ping, Cheng Jing-Qui, Lu Yan-Rong, Long Dan
Lab of Transplantation Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Nov;36(6):770-2.
To detect the integration and expression of porcine endogenous retrovirus (PERV) in the immortal cell line of Banna Minipig Inbred Line-Mesenchymal Stem Cells (BMI-MSCs).
DNA and total RNA of the immortal cell line of BMI-MSCs were extracted and PCR, RT-PCR were performed to detect PERV-gag, pol and env gene, and the type of PERV was also detected.
PERV-gag, pol and env gene were all detected in the primary culture and immortal cell line (passage 150 and passage 180) of BMI-MSCs, and the type of PERV was PERV-A, B. Functional expression of PERV-gag and pol mRNA was also detected.
In this laboratory, PERV was not lost during the proceeding of pig inbred and since has been in long-term culture of pig cells in vitro. PERV has integrated into the genome of its natural host, and virus mRNA can effectively express. So it is very essential to evaluate the possibility of xenozoonoses in pig-to-human xenotransplantation.
检测猪内源性逆转录病毒（PERV）在版纳微型猪近交系间充质干细胞（BMI-MSCs）永生化细胞系中的整合与表达情况。
提取BMI-MSCs永生化细胞系的DNA和总RNA，进行PCR、RT-PCR检测PERV-gag、pol和env基因，并检测PERV的类型。
在BMI-MSCs原代培养物及永生化细胞系（第150代和第180代）中均检测到PERV-gag、pol和env基因，PERV类型为PERV-A、B型。还检测到PERV-gag和pol mRNA的功能性表达。
在本实验室中，PERV在猪近交过程中未丢失，且在猪细胞体外长期培养过程中一直存在。PERV已整合到其天然宿主的基因组中，病毒mRNA能够有效表达。因此，评估猪到人的异种移植中发生人畜共患病的可能性非常必要。
