[版纳微型猪近交系基因组中猪内源性逆转录病毒拷贝数的定量研究]
[Quantitative study on copy numbers of porcine endogenous retroviruses in genome of Banna miniature swine inbred line].
作者信息
Huang Xiao-jie, Chen Ping, Zeng Rong, Ma Kang-tao, Zeng Yang-zhi, Zhou Chun-yan
机构信息
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Aug 18;38(4):370-4.
OBJECTIVE
To investigate the copy numbers of porcine endogenous retroviruses in genome of Banna miniature swines for screening of donors in xenotransplantation with porcine transplants.
METHODS
The genomic DNA of peripheral blood mononuclear cells(PBMCs) of 50 Banna miniature swines in 4 inbred sublines and 5 European Large White Pigs was extracted for the detection to pol, envA, envB, envC and envA/C recombinant in PERVs with real-time quantitative/semi-quantitative PCR and normal PCR.
RESULTS
PCR products of pol, envA and envB of PERV could be obtained from all the Banna samples, and the mean amount of these products was 24.2+/-9.4,13.1+/-8.4,16.4+/-9.8 respectively and was significantly different among the inbred herds (P<0.01). The envC and envA/C were not amplified.
CONCLUSION
The copy numbers of pol, envA and envB genes of PERV in porcine genome were significantly different among the inbred herds of Banna miniature swines, and envC and envA/C were absent in genome of Banna Miniature Swines.
目的
检测版纳微型猪基因组中猪内源性逆转录病毒（PERV）的拷贝数，为猪异种移植供体筛选提供依据。
方法
采集4个近交系版纳微型猪50份外周血单个核细胞（PBMC）基因组DNA和5份欧洲大白猪基因组DNA，采用实时定量/半定量PCR和普通PCR检测PERV的pol、envA、envB、envC及envA/C重组基因。
结果
所有版纳微型猪样本均能扩增出PERV的pol、envA和envB基因，其平均拷贝数分别为24.2±9.4、13.1±8.4、16.4±9.8，不同近交群体间差异有统计学意义（P<0.01）。未扩增出envC和envA/C基因。
结论
版纳微型猪不同近交群体基因组中PERV的pol、envA和envB基因拷贝数存在显著差异，且基因组中不存在envC和envA/C基因。
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