[Ⅰ型胶原酶体外酶解人牙髓细胞的研究]
[Study on enzymatically released human dental pulp cells with collagenase I in vitro].
作者信息
Wang Feng-Ming, Hu Tao, Zhou Xue-Dong
机构信息
Key Laboratory of Oral Biomedical Engineering Ministry of Education, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Nov;36(6):877-9.
OBJECTIVE
To investigate the alkaline phosphatase activity of enzymatically released human dental pulp cells with collagenase I in vitro.
METHODS
We cultivated human dental pulp cells from connective tissue explants digested with collagenase I. Immunocytochemical staining was performed for characterization. When the subcultured cells grew in multilayers, the activity of alkaline phosphatase was examined by enzyme histochemical staining,and the ability of mineralization was detected.
RESULTS
Cultures of human primary dental pulp cells became confluent after 15-20 days. Subcultured cells proliferated to multilayers in long-term cultures, and the staining of alkaline phosphatase was noted to be regional on culture slides. The pulp cells cultured in the presence of beta-glycerophosphate and L-ascorbic acid formed mineralization nodules.
CONCLUSION
These results suggest that human dental pulp cells can be cultivated preferably from tissue explants digested with collagenase I in vitro. Enzyme histochemistry is useful for studying the biological behavior of dental pulp cells.
目的
体外研究用I型胶原酶酶解释放的人牙髓细胞的碱性磷酸酶活性。
方法
我们从用I型胶原酶消化的结缔组织外植体中培养人牙髓细胞。进行免疫细胞化学染色以进行表征。当传代培养的细胞长成多层时，通过酶组织化学染色检测碱性磷酸酶的活性，并检测矿化能力。
结果
人原代牙髓细胞培养物在15 - 20天后汇合。传代培养的细胞在长期培养中增殖形成多层，并且在培养玻片上观察到碱性磷酸酶染色呈区域性。在β-甘油磷酸酯和L-抗坏血酸存在下培养的牙髓细胞形成矿化结节。
结论
这些结果表明，人牙髓细胞可以较好地从体外经I型胶原酶消化的组织外植体中培养出来。酶组织化学对于研究牙髓细胞的生物学行为是有用的。
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