Panagakos F S
Department of Prosthodontics and Biomaterials, New Jersey Dental School, Newark 07103, USA.
J Endod. 1998 Mar;24(3):171-5. doi: 10.1016/S0099-2399(98)80177-5.
The odontoblast is the cell responsible for dentin formation and mineralization during tooth development. A number of primary pulp cell culture systems have been used to study the mechanism of dentinogenesis in vitro. One of the difficulties in using primary cells is the limited number of cell divisions they will undergo. In this study, this problem was addressed by transfecting primary cultures of human pulp cells with an SV40-adenovirus construct. This resulted in the establishment of transformed human pulp cells, which were named HPC-T. A series of preliminary experiments were performed to characterize these cells, including their morphology, cell proliferation, alkaline phosphatase production, and cytogenetic make-up. The results demonstrate that SV40-transformed human pulp cells retain many of the characteristics of the parent primary cells and may be useful in the study of pulp cell function in vitro.
成牙本质细胞是在牙齿发育过程中负责牙本质形成和矿化的细胞。许多原代牙髓细胞培养系统已被用于体外研究牙本质形成的机制。使用原代细胞的困难之一是它们所能经历的细胞分裂次数有限。在本研究中，通过用SV40-腺病毒构建体转染人牙髓细胞的原代培养物解决了这个问题。这导致了转化的人牙髓细胞的建立，它们被命名为HPC-T。进行了一系列初步实验来表征这些细胞，包括它们的形态、细胞增殖、碱性磷酸酶产生和细胞遗传学组成。结果表明，SV40转化的人牙髓细胞保留了许多亲代原代细胞的特征，可能有助于体外牙髓细胞功能的研究。
