Hospodar'ov D V, Mandryk S Ia, Lushchak V I
Ukr Biokhim Zh (1999). 2005 Mar-Apr;77(2):162-5.
The content of protein carbonyls and thiobarbituric acid reactive substances (TBARS) in the wild and catalase-deficient strains of the yeast Saccharomyces cerevisiae grown in glucose and ethanol media are compared. The deficient strain cells reproduced 10.6-fold slower in ethanol-containing medium. Activity of glucose-6-phosphate dehydrogenase in YWT1 cells was 1.7-fold lower when yeast are grown in ethanol, and content of protein carbonyls was 4.7-fold higher, than when they are grown in the medium with glucose. At the same time, reproduction of the wild type cells in ethanol was 2.7-fold slower and carbonyl groups of protein content was 2-fold lower, than under cultivation in glucose. TBARS content in both strains was similar when they were grown in ethanol and in glucose. It has been supposed that catalases play a certain role in the protection of S. cerevisiae proteins against oxidative modification when they are grown on the media with glucose and ethanol.
比较了在葡萄糖和乙醇培养基中生长的野生型和过氧化氢酶缺陷型酿酒酵母菌株中蛋白质羰基和硫代巴比妥酸反应性物质（TBARS）的含量。缺陷型菌株细胞在含乙醇的培养基中繁殖速度慢10.6倍。当酵母在乙醇中生长时，YWT1细胞中葡萄糖-6-磷酸脱氢酶的活性比在葡萄糖培养基中生长时低1.7倍，蛋白质羰基含量高4.7倍。同时，野生型细胞在乙醇中的繁殖速度比在葡萄糖中培养时慢2.7倍，蛋白质羰基含量低2倍。当两种菌株在乙醇和葡萄糖中生长时，TBARS含量相似。据推测，过氧化氢酶在酿酒酵母在葡萄糖和乙醇培养基上生长时保护其蛋白质免受氧化修饰方面发挥一定作用。
