Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237, Łódź, Poland.
Cell Mol Biol Lett. 2007 Sep;12(3):448-56. doi: 10.2478/s11658-007-0017-y. Epub 2007 May 10.
We compared the oxidation of dihydrorhodamine 123, glutathione contents and activities of superoxide dismutase (SOD) and catalase for three wild-type strains of Saccharomyces cerevisiae grown on media with different carbon sources. The rate of oxidation of dihydrorhodamine 123 was much higher in respiring cells grown on ethanol or glycerol media than in fermenting cells grown on glucose medium. The total SOD activity was highest on glycerol medium and lowest on ethanol medium, while the catalase activity was highest on glycerol medium. The sequence of glutathione content values was: glucose > ethanol > glycerol.
我们比较了三种不同碳源培养基中生长的野生型酿酒酵母细胞的二氢罗丹明 123 氧化率、谷胱甘肽含量以及超氧化物歧化酶(SOD)和过氧化氢酶的活性。在乙醇或甘油培养基中生长的呼吸细胞中二氢罗丹明 123 的氧化速率远高于在葡萄糖培养基中生长的发酵细胞。总 SOD 活性在甘油培养基中最高,在乙醇培养基中最低,而过氧化氢酶活性在甘油培养基中最高。谷胱甘肽含量值的顺序为:葡萄糖>乙醇>甘油。