Semchyshyn H, Bagnyukova T, Lushchak V
Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, 76025, Ukraine.
Biochemistry (Mosc). 2005 Nov;70(11):1238-44. doi: 10.1007/s10541-005-0253-6.
The effect of hydrogen peroxide on the activity of soxRS and oxyR regulon enzymes in different strains of Escherichia coli has been studied. Treatment of bacteria with 20 microM H2O2 caused an increase in catalase and peroxidase activities (oxyR regulon) in all strains investigated. It is shown for the first time that oxidative stress induced by hydrogen peroxide causes in some E. coli strains a small increase in activity of superoxide dismutase and glucose-6-phosphate dehydrogenase (soxRS regulon). This effect is cancelled by chloramphenicol, an inhibitor of protein synthesis in prokaryotes. The increase in soxRS regulon enzyme activities was not found in the strain lacking the soxR gene. These results provide evidence for the involvement of the soxRS regulon in the adaptive response of E. coli to oxidative stress induced by hydrogen peroxide.
研究了过氧化氢对不同大肠杆菌菌株中soxRS和oxyR调控子酶活性的影响。用20微摩尔过氧化氢处理细菌，导致所有研究菌株中的过氧化氢酶和过氧化物酶活性（oxyR调控子）增加。首次表明，过氧化氢诱导的氧化应激在一些大肠杆菌菌株中导致超氧化物歧化酶和葡萄糖-6-磷酸脱氢酶（soxRS调控子）活性略有增加。这种效应被氯霉素（一种原核生物蛋白质合成抑制剂）消除。在缺乏soxR基因的菌株中未发现soxRS调控子酶活性增加。这些结果为soxRS调控子参与大肠杆菌对过氧化氢诱导的氧化应激的适应性反应提供了证据。
