过氧化氢在体内激活SoxRS调节子。
Hydrogen peroxide activates the SoxRS regulon in vivo.
作者信息
Manchado M, Michán C, Pueyo C
机构信息
Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071 Córdoba, Spain.
出版信息
J Bacteriol. 2000 Dec;182(23):6842-4. doi: 10.1128/JB.182.23.6842-6844.2000.
Abstract
By multiplex reverse transcription-PCR, we demonstrate that the SoxRS response, which protects cells against superoxide toxicity, is triggered also by hydrogen peroxide. SoxR-dependent inductions of 7. 3-, 7.6-, 4.6-, 2.2-, and 2.6-fold were quantified for soxS, micF, sodA, inaA, and fpr transcripts, respectively. This finding suggests an extensive and tight connectivity between different regulatory pathways in the Escherichia coli response to oxidative stress.
摘要
通过多重逆转录聚合酶链反应，我们证明了保护细胞免受超氧化物毒性的SoxRS应答也可由过氧化氢触发。分别对soxS、micF、sodA、inaA和fpr转录本进行了SoxR依赖性诱导定量，诱导倍数分别为7.3倍、7.6倍、4.6倍、2.2倍和2.6倍。这一发现表明，在大肠杆菌对氧化应激的应答中，不同调控途径之间存在广泛而紧密的联系。
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