Fu Qiang, Wang Bai-Chen, Jin Xiang, Li Hong-Bing, Han Pei, Wei Kai-hua, Zhang Xue-min, Zhu Yu-Xian
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
J Biochem Mol Biol. 2005 Nov 30;38(6):650-60. doi: 10.5483/bmbrep.2005.38.6.650.
Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3 % of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.
对干燥、分层的拟南芥种子或幼苗中积累的蛋白质（根据取样时间不同，总量为1100至1300），采用固定化pH梯度双向电泳进行分析。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）或飞行时间串联质谱（TOF-TOF）确定了由355个独立基因编码的437种多肽的分子身份。总体而言，293种多肽在所有阶段均存在，95种在胚根突出时积累，另有18种出现在后期阶段。进一步分析表明，已鉴定的多肽中有226种可定位到不同的代谢途径。参与碳水化合物、能量和氨基酸代谢的蛋白质约占1/4，参与维生素和辅因子代谢的蛋白质约占从72小时幼苗制备的凝胶中总信号强度的3%。与遗传信息处理相关的酶在早期吸水过程中迅速增加，并在萌发约30小时时达到最高水平。
