Figueiró S D, Macêdo A A M, Melo M R S, Freitas A L P, Moreira R A, de Oliveira R S, Góes J C, Sombra A S B
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Biophys Chem. 2006 Mar 20;120(2):154-9. doi: 10.1016/j.bpc.2005.11.001. Epub 2005 Dec 5.
In this paper, impedance measurements in the frequency range from 10(-2) to 10(6) Hz are presented for collagen and algal sulfated polysaccharide crosslinked films. We are considering the development of new biomaterials which have potential applications in coating of cardiovascular prostheses, support for cellular growth and in systems for controlled drug delivery. The effect of crosslink sulfated polysaccharide on the physical chemical properties of collagen was studied using FT-infrared spectroscopy, differential scanning calorimetry (DSC), dielectric spectroscopy. The resulting films crosslinked with glutaraldehyde (GA) in concentrations of 0.001% and 0.05% when analysed by DSC, showed that the GA treatment not only left the thermal stability of the collagen unaffected, but it also decreased the thermal transition energy. Dielectric spectroscopy shows that the effect of the crosslink on the blend film was associated to the decrease and stabilization of the dielectric permittivity at low frequencies and decreased its conductivity.
本文介绍了胶原蛋白和藻类硫酸化多糖交联膜在10⁻²至10⁶Hz频率范围内的阻抗测量结果。我们正在考虑开发新型生物材料，这些材料在心血管假体涂层、细胞生长支持以及控释药物系统中具有潜在应用。使用傅里叶变换红外光谱、差示扫描量热法（DSC）和介电谱研究了交联硫酸化多糖对胶原蛋白物理化学性质的影响。通过DSC分析，用浓度为0.001%和0.05%的戊二醛（GA）交联得到的薄膜表明，GA处理不仅使胶原蛋白的热稳定性不受影响，而且还降低了热转变能。介电谱表明，交联对共混膜的影响与低频下介电常数的降低和稳定以及电导率的降低有关。
