Tissue Engineering Group, Department of Biomedical Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
Department of Oral and Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
Int Wound J. 2023 May;20(5):1566-1577. doi: 10.1111/iwj.14012. Epub 2022 Nov 13.
Biological matrices can be modified with cross-linkers to improve some of their characteristics as scaffolds for tissue engineering. In this study, chemical cross-linker 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was used with different ratios (5, 10, 20, 30, and 40 mM) to improve properties such as mechanical strength, denaturation temperature, and degradability of the acellular fish skin as a biological scaffold for tissue engineering applications. Morphological analysis showed that the use of cross-linker at low concentrations had no effect on the structure and textiles of the scaffold, while increasing mechanical strength, denaturation temperature, and degradation time. Cytotoxicity and cellular studies showed that the optimal cross-linker concentration did not significantly affect cell viability as well as cell adhesion. In general, utilising the carbodiimide cross-linker with the optimal ratio can improve the characteristics and function of the biological tissues such as acellular fish skin.
生物基质可以用交联剂进行修饰，以改善其作为组织工程支架的某些特性。在这项研究中，使用了化学交联剂 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺（EDC），并采用不同的比例（5、10、20、30 和 40mM）来改善脱细胞鱼皮的机械强度、变性温度和降解性等特性，将其作为组织工程应用的生物支架。形态分析表明，交联剂低浓度的使用对支架的结构和质地没有影响，同时提高了机械强度、变性温度和降解时间。细胞毒性和细胞研究表明，最佳交联剂浓度对细胞活力和细胞黏附没有显著影响。总的来说，使用最佳比例的碳二亚胺交联剂可以改善生物组织（如脱细胞鱼皮）的特性和功能。
