核糖体高特异性核糖毒素识别的建模

Modeling the highly specific ribotoxin recognition of ribosomes.

作者信息

García-Mayoral Flor, García-Ortega Lucía, Alvarez-García Elisa, Bruix Marta, Gavilanes José G, del Pozo Alvaro Martínez

机构信息

Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain.

出版信息

FEBS Lett. 2005 Dec 19;579(30):6859-64. doi: 10.1016/j.febslet.2005.11.027. Epub 2005 Dec 1.

DOI:10.1016/j.febslet.2005.11.027

PMID:16337202

Abstract

The three-dimensional structures of the alpha-sarcin ribotoxin and its delta(7-22) deletion mutant, both complexed with a 20-mer oligonucleotide mimicking the sarcin/ricin loop (SRL) of the ribosome, have been docked into the structure of the Halobacterium marismortui ribosome by fitting the nucleotide atomic coordinates into those of the ribosomal SRL. This study has revealed that two regions of the ribotoxin, residues 11-16 and 84-85, contact the ribosomal proteins L14 (residues 99-105) and L6 (residues 88-92), respectively. The first of these two ribotoxin regions appears to be crucial for its specific ribosome recognition.

摘要

已通过将核苷酸原子坐标与核糖体的肌动蛋白/蓖麻毒素环（SRL）的20聚体寡核苷酸模拟物拟合，将与核糖体SRL模拟物复合的α-肌动蛋白核糖毒素及其δ（7-22）缺失突变体的三维结构对接至嗜盐栖热袍菌核糖体的结构中。这项研究表明，核糖毒素的两个区域，即残基11-16和84-85，分别与核糖体蛋白L14（残基99-105）和L6（残基88-92）接触。这两个核糖毒素区域中的第一个似乎对其特异性核糖体识别至关重要。