Martínez Mar, Bernal Pilar, Almela Concepción, Vélez Dinoraz, García-Agustín Pilar, Serrano Ramón, Navarro-Aviñó Juan
Department of the Stress Biology, IBMCP, CSIC, Camino de Vera s.n., Post Code 46022 Valencia, Spain.
Chemosphere. 2006 Jun;64(3):478-85. doi: 10.1016/j.chemosphere.2005.10.044. Epub 2005 Dec 7.
Nicotiana glauca transformed with TaPCS1 was tested for its application in phytoremediation. When plantlets were grown in mine soils containing Cu, Zn, and Pb (42, 2600, and 1500 mg kg(-1)) the plant showed high levels of accumulation especially of Zn and Pb. Adult plants growing in mine soils containing different heavy metal concentrations showed a greater accumulation as well as an extension to a wider range of elements, including Cd, Ni and B. The overexpressed gene confers up to 9 and 36 times more Cd and Pb accumulation in the shoots under hydroponic conditions, and a 3- and 6-fold increase in mining soils. When the hyperaccumulator Thlaspi caerulescens was compared, the results were higher values of heavy metal and Boron accumulation, with a yield of 100 times more biomass. Thlaspi was unable to survive in mining soils containing either a level higher than 11000 mg kg(-1) of Pb and 4500 mg kg(-1) of Zn, while engineered plants yielded an average of 0.5 kg per plant.
对转入TaPCS1基因的黄花烟草进行了植物修复应用测试。当幼苗在含有铜、锌和铅（分别为42、2600和1500毫克/千克）的矿质土壤中生长时，该植物表现出较高的积累水平，尤其是锌和铅。在含有不同重金属浓度的矿质土壤中生长的成年植株也表现出更大的积累量，并且积累元素范围更广，包括镉、镍和硼。在水培条件下，过表达的基因使地上部镉和铅的积累量分别增加多达9倍和36倍，在矿质土壤中增加3倍和6倍。与超积累植物天蓝遏蓝菜相比，转基因植物的重金属和硼积累量更高，生物量产量高出100倍。天蓝遏蓝菜在铅含量高于11000毫克/千克和锌含量高于4500毫克/千克的矿质土壤中无法存活，而转基因植物单株平均产量为0.5千克。
