Cortès Sandra, Glade Nicolas, Chartier Isabelle, Tabony James
Commissariat à l'Energie Atomique, Département Réponse et Dynamique Cellulaires, Laboratoire d'Immunochimie, INSERM U548, D.S.V, C.E.A. Grenoble, 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
Biophys Chem. 2006 Apr 1;120(3):168-77. doi: 10.1016/j.bpc.2005.11.002. Epub 2005 Dec 7.
Under appropriate conditions, in vitro microtubule preparations self-organise over macroscopic distances by a process of reaction and diffusion. To investigate whether such self-organisation can also occur in objects as small as a cell or an embryo we carried out experiments in miniature containers of cellular dimension. When assembled under self-organising conditions in wells of 120-500 microm, microtubules developed organised structures. Self-organisation is strongly affected by shape, being highly favoured by elongated forms. In wells of more complex shape, geometrical factors may either oppose or strengthen one another and so inhibit or reinforce self-organisation. Microtubules were also assembled within phospholipid vesicles of 2-5 microm diameter. Under self-organising conditions, we observed large shape changes from spheroids to long tubes (50-100 microm) and intertwined coils. We conclude that self-organisation of microtubules by reaction-diffusion processes can occur in containers of cellular dimensions and is capable of strongly deforming the cellular membrane.
在适当条件下，体外微管制剂通过反应和扩散过程在宏观距离上自我组织。为了研究这种自我组织是否也能在细胞或胚胎那么小的物体中发生，我们在细胞尺寸的微型容器中进行了实验。当在120 - 500微米的孔中在自我组织条件下组装时，微管形成了有组织的结构。自我组织受到形状的强烈影响，细长形式非常有利于自我组织。在形状更复杂的孔中，几何因素可能相互对抗或相互加强，从而抑制或加强自我组织。微管也在直径为2 - 5微米的磷脂囊泡内组装。在自我组织条件下，我们观察到从球体到长管（50 - 100微米）以及缠绕线圈的巨大形状变化。我们得出结论，通过反应扩散过程进行的微管自我组织可以在细胞尺寸的容器中发生，并且能够使细胞膜发生强烈变形。
