Wakana Kimio, Akiyama Yoshimitsu, Aso Takeshi, Yuasa Yasuhito
Department of Comprehensive Reproductive Medicine, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.
Cancer Lett. 2006 Sep 28;241(2):281-8. doi: 10.1016/j.canlet.2005.10.039. Epub 2005 Dec 7.
To clarify the role of GATA transcription factors in ovarian carcinogenesis, we analyzed the expression and methylation states of GATA-4/-5/-6 in eight human ovarian cancer cell lines. GATA-4/-5 were methylated in three and two cell lines without their expression, respectively. Methylation of GATA-4/-5 was also detected in nine and five of 15 primary ovarian cancers, respectively. GATA-6 was not methylated in any cases. We transiently over-expressed GATA-5 in the JHOC-5 cell line using an adenovirus system, resulting in that apoptosis was induced and apoptosis-related genes, such as Apaf-1, were up-regulated. These data suggest that GATA-4/-5 may be involved in ovarian carcinogenesis.
为阐明GATA转录因子在卵巢癌发生中的作用，我们分析了8种人卵巢癌细胞系中GATA-4/-5/-6的表达及甲基化状态。GATA-4/-5分别在3种和2种细胞系中发生甲基化且无表达。在15例原发性卵巢癌中，分别有9例和5例检测到GATA-4/-5甲基化。所有病例中GATA-6均未发生甲基化。我们使用腺病毒系统在JHOC-5细胞系中瞬时过表达GATA-5，结果诱导了细胞凋亡，且上调了凋亡相关基因如Apaf-1的表达。这些数据表明GATA-4/-5可能参与了卵巢癌的发生。
