Volk Sara, Wang Min, Pickart Cecile M
Department of Biochemistry and Molecular Biology, Johns Hopkins University, Baltimore, Maryland, USA.
Methods Enzymol. 2005;399:3-20. doi: 10.1016/S0076-6879(05)99001-0.
Ubiquitin can be conjugated to lysine residues of other ubiquitin molecules to form polymers called polyubiquitin chains. Ubiquitin has seven lysine residues, creating the potential for seven distinct types of chains, at least five of which have been observed in vitro or in vivo. A subset of these chains mediates substrate targeting to proteasomes, whereas other types of chains have been implicated in nonproteolytic signaling pathways. In this chapter, we outline chemical and genetic strategies that can be used to deduce (or control) the structures of polyubiquitin chains in vitro and in living cells.
泛素可以与其他泛素分子的赖氨酸残基结合，形成称为多聚泛素链的聚合物。泛素有七个赖氨酸残基，这使得有可能形成七种不同类型的链，其中至少五种已在体外或体内观察到。这些链的一个子集介导底物靶向蛋白酶体，而其他类型的链则与非蛋白水解信号通路有关。在本章中，我们概述了可用于在体外和活细胞中推断（或控制）多聚泛素链结构的化学和遗传策略。
