Valdés G, Neves L A A, Anton L, Corthorn J, Chacón C, Germain A M, Merrill D C, Ferrario C M, Sarao R, Penninger J, Brosnihan K B
Departamento Nefrología, Facultad Medicina Universidad Católica Santiago, Chile.
Placenta. 2006 Feb-Mar;27(2-3):200-7. doi: 10.1016/j.placenta.2005.02.015. Epub 2005 Apr 25.
This work was designed to study the expression of the vasodilator peptide angiotensin-(1-7) [Ang-(1-7)] and its generating enzyme (ACE2) in the uteroplacental interface. Placentas were obtained from 11 early pregnancy failures (5 miscarriages and 6 ectopic pregnancies), 15 normotensive, and 10 preeclamptic gestations. In placental villi, the main sites of immunocytochemical expression of Ang-(1-7) and ACE2 were the syncytiotrophoblast, cytotrophoblast, endothelium and vascular smooth muscle of primary and secondary villi. Syncitial Ang-(1-7) expression in samples obtained from miscarriages and ectopic pregnancies was increased compared to normal term pregnancy [2.0 (2.0-2.25 for the 25 and 75% interquartile range) vs 1.3 (1.0-1.9), p<0.01]. In the maternal stroma, Ang-(1-7) and ACE2 were expressed in the invading and intravascular trophoblast and in decidual cells in all 3 groups. Ang-(1-7) and ACE2 staining was also found in arterial and venous endothelium and smooth muscle of the umbilical cord. The expression of Ang-(1-7) and ACE2 was similar in samples obtained from normal term or preeclamptic pregnancies, except for increased expression of ACE2 in umbilical arterial endothelium in preeclampsia [0.5 (0.5-0.8) vs 0.0 (0.0-0.0), p<0.01]. The uteroplacental location of Ang-(1-7) and ACE2 in pregnancy suggests an autocrine function of Ang-(1-7) in the vasoactive regulation that characterizes placentation and established pregnancy.
本研究旨在探讨血管舒张肽血管紧张素-(1-7)[Ang-(1-7)]及其生成酶(ACE2)在子宫胎盘界面的表达。研究收集了11例早期妊娠失败病例(5例流产和6例异位妊娠)、15例血压正常孕妇以及10例先兆子痫孕妇的胎盘组织。在胎盘绒毛中，Ang-(1-7)和ACE2免疫细胞化学表达的主要部位是合体滋养层、细胞滋养层、初级和次级绒毛的内皮细胞及血管平滑肌。与正常足月妊娠相比，流产和异位妊娠样本中的合体Ang-(1-7)表达增加[四分位间距25%和75%时分别为2.0(2.0 - 2.25) vs 1.3(1.0 - 1.9)，p<0.01]。在母体基质中，三组样本的侵袭性和血管内滋养层细胞以及蜕膜细胞均表达Ang-(1-7)和ACE2。在脐带的动脉和静脉内皮及平滑肌中也发现了Ang-(1-7)和ACE2染色。除先兆子痫患者脐动脉内皮中ACE2表达增加外[0.5(0.5 - 0.8) vs 0.0(0.0 - 0.0)，p<0.01]，正常足月妊娠和先兆子痫妊娠样本中Ang-(1-7)和ACE2的表达相似。妊娠时子宫胎盘部位的Ang-(1-7)和ACE2表明Ang-(1-7)在胎盘形成和妊娠建立过程中血管活性调节方面具有自分泌功能。
