Costa A M A, Maximiano E B B, Avvad-Portari E, Jésus N R, Levy R A, Porto L C
Histology and Embryology Department, State University of Rio de Janeiro, Rua Prof Manoel de Abreu, 20550-170 Rio de Janeiro RJ, Brazil.
Placenta. 2006 Feb-Mar;27(2-3):234-43. doi: 10.1016/j.placenta.2005.02.018.
Placentae from patients with preeclampsia (PE) and systemic lupus erythematosus (SLE) present many alterations that may impair materno-fetal exchange. We investigated the distribution of contractile cells and fibrillin-1 in terminal villi of term placentae from patients with PE or SLE and compared to control placentae. Stroma in terminal villi exhibited intense labelling for fibrillin-1. The fibrillin-1 villi surface fraction was greater in PE and SLE placentae than in controls (13+/-0.4%, 14+/-0.5%, 10+/-0.4%; p=0.0001). Immunohistochemistry for alpha-smooth muscle (SM) actin showed few contractile cells in control terminal villi stroma, localized around fetal capillaries and showed rare processes in vasculo-syncytial membrane. PE and SLE placentae exhibited an increase in the number of capillaries presenting alpha-SM actin adventitial positive cells. The presence of alpha-SM actin processes interposed in the vasculo-syncytial membrane was greater in SLE villi than in PE and controls. Ultrastructural observations confirmed in SLE and PE terminal villi the presence of these processes in vasculo-syncytial membrane and also showed a thickened trophoblastic basement membrane. The present study demonstrates that an important myofibroelastic system is present in term terminal villi, and that this system is actively remodelled in PE and SLE placentae.
子痫前期（PE）和系统性红斑狼疮（SLE）患者的胎盘存在许多可能损害母胎交换的改变。我们研究了PE或SLE患者足月胎盘终末绒毛中收缩细胞和原纤维蛋白-1的分布，并与对照胎盘进行比较。终末绒毛中的基质对原纤维蛋白-1显示出强烈的标记。PE和SLE胎盘的原纤维蛋白-1绒毛表面分数高于对照组（13±0.4%、14±0.5%、10±0.4%；p = 0.0001）。α-平滑肌（SM）肌动蛋白的免疫组织化学显示对照终末绒毛基质中收缩细胞很少，位于胎儿毛细血管周围，在血管合体膜中显示罕见的突起。PE和SLE胎盘显示出现α-SM肌动蛋白外膜阳性细胞的毛细血管数量增加。SLE绒毛中插入血管合体膜的α-SM肌动蛋白突起比PE和对照组更多。超微结构观察在SLE和PE终末绒毛中证实了血管合体膜中存在这些突起，并且还显示滋养层基底膜增厚。本研究表明足月终末绒毛中存在一个重要的肌纤维弹性系统，并且该系统在PE和SLE胎盘中被积极重塑。
