去甲肾上腺素在下丘脑的谷氨酸能突触处引发多泡释放。
Noradrenaline triggers multivesicular release at glutamatergic synapses in the hypothalamus.
作者信息
Gordon Grant R J, Bains Jaideep S
机构信息
Hotchkiss Brain Institute, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, T2N 4N1, Canada.
出版信息
J Neurosci. 2005 Dec 7;25(49):11385-95. doi: 10.1523/JNEUROSCI.2378-05.2005.
Abstract
The origin of large-amplitude miniature EPSCs (mEPSCs) at central synapses remains to be firmly established. Here, we show that at excitatory synapses onto magnocellular neurosecretory cells in the hypothalamus, noradrenaline induces a rapid and robust increase in mEPSC amplitude that requires alpha1-adrenoceptor activation but is impervious to postsynaptic manipulations that block the putative insertion of AMPA receptors. In response to noradrenaline, mEPSCs exhibit a putative multimodal amplitude histogram distribution that is not attributable to random temporal summation, the unveiling of a quiescent synapse, or the release of large vesicles. Large-amplitude mEPSCs are sensitive to a high dose of ryanodine and are associated with an enhanced glutamate cleft concentration. Together, these data are consistent with the hypothesis that large-amplitude mEPSCs result from the synchronous release of multiple vesicles via rapid presynaptic calcium expulsion from intracellular stores.
摘要
中枢突触处大幅度微小兴奋性突触后电流（mEPSCs）的起源仍有待确切证实。在此，我们表明，在下丘脑大细胞神经分泌细胞的兴奋性突触处，去甲肾上腺素可诱导mEPSC幅度迅速且显著增加，这需要α1 - 肾上腺素能受体激活，但不受阻断AMPA受体假定插入的突触后操作的影响。对去甲肾上腺素的反应中，mEPSCs呈现出一种假定的多峰幅度直方图分布，这并非归因于随机的时间总和、静息突触的显露或大囊泡的释放。大幅度mEPSCs对高剂量的ryanodine敏感，且与谷氨酸裂隙浓度增加有关。总之，这些数据与以下假设一致：大幅度mEPSCs是通过细胞内钙库中突触前钙的快速排出导致多个囊泡同步释放的结果。
相似文献
1
Noradrenaline triggers multivesicular release at glutamatergic synapses in the hypothalamus.去甲肾上腺素在下丘脑的谷氨酸能突触处引发多泡释放。
J Neurosci. 2005 Dec 7;25(49):11385-95. doi: 10.1523/JNEUROSCI.2378-05.2005.
2
Giant miniature EPSCs at the hippocampal mossy fiber to CA3 pyramidal cell synapse are monoquantal.海马苔藓纤维至CA3锥体细胞突触处的巨型微小兴奋性突触后电流是单量子的。
J Neurophysiol. 2002 Jan;87(1):15-29. doi: 10.1152/jn.00394.2001.
3
Developmental increase in vesicular glutamate content does not cause saturation of AMPA receptors at the calyx of Held synapse.囊泡谷氨酸含量的发育性增加不会导致Held壶腹突触处AMPA受体饱和。
J Neurosci. 2003 May 1;23(9):3633-8. doi: 10.1523/JNEUROSCI.23-09-03633.2003.
4
Priming of excitatory synapses by alpha1 adrenoceptor-mediated inhibition of group III metabotropic glutamate receptors.α1肾上腺素能受体介导的Ⅲ型代谢型谷氨酸受体抑制对兴奋性突触的启动作用。
J Neurosci. 2003 Jul 16;23(15):6223-31. doi: 10.1523/JNEUROSCI.23-15-06223.2003.
5
The speeding of EPSC kinetics during maturation of a central synapse.中枢突触成熟过程中兴奋性突触后电流动力学的加速。
Eur J Neurosci. 2002 Mar;15(5):785-97. doi: 10.1046/j.1460-9568.2002.01910.x.
6
The origin of quantal size variation: vesicular glutamate concentration plays a significant role.量子大小变化的起源：囊泡谷氨酸浓度起着重要作用。
J Neurosci. 2007 Mar 14;27(11):3046-56. doi: 10.1523/JNEUROSCI.4415-06.2007.
7
BDNF enhances quantal neurotransmitter release and increases the number of docked vesicles at the active zones of hippocampal excitatory synapses.脑源性神经营养因子可增强量子神经递质释放，并增加海马兴奋性突触活动区对接囊泡的数量。
J Neurosci. 2001 Jun 15;21(12):4249-58. doi: 10.1523/JNEUROSCI.21-12-04249.2001.
8
Quantal mEPSCs and residual glutamate: how horizontal cell responses are shaped at the photoreceptor ribbon synapse.量子化微小兴奋性突触后电流与残余谷氨酸：水平细胞反应在光感受器带状突触处是如何形成的。
Eur J Neurosci. 2008 May;27(10):2575-86. doi: 10.1111/j.1460-9568.2008.06226.x.
9
Impact of vesicular glutamate leakage on synaptic transmission at the calyx of Held.囊泡谷氨酸泄漏对Held壶腹突触传递的影响。
J Physiol. 2017 Feb 15;595(4):1263-1271. doi: 10.1113/JP273467. Epub 2016 Nov 29.
10
Properties of quantal transmission at CA1 synapses.CA1 突触处量子传递的特性。
J Neurophysiol. 2004 Oct;92(4):2456-67. doi: 10.1152/jn.00258.2004. Epub 2004 Apr 28.
引用本文的文献
1
Estimating the Readily-Releasable Vesicle Pool Size at Synaptic Connections in the Neocortex.估算新皮层突触连接中易释放囊泡池的大小。
Front Synaptic Neurosci. 2019 Oct 15;11:29. doi: 10.3389/fnsyn.2019.00029. eCollection 2019.
2
Noradrenaline Increases mEPSC Frequency in Pyramidal Cells in Layer II of Rat Barrel Cortex via Calcium Release From Presynaptic Stores.去甲肾上腺素通过突触前储存部位释放钙来增加大鼠桶状皮层II层锥体神经元微小兴奋性突触后电流的频率。
Front Cell Neurosci. 2018 Jul 27;12:213. doi: 10.3389/fncel.2018.00213. eCollection 2018.
3
Drosophila CaV2 channels harboring human migraine mutations cause synapse hyperexcitability that can be suppressed by inhibition of a Ca2+ store release pathway.果蝇 Cav2 通道携带人类偏头痛突变导致突触过度兴奋，可通过抑制钙库释放途径来抑制。
PLoS Genet. 2018 Aug 6;14(8):e1007577. doi: 10.1371/journal.pgen.1007577. eCollection 2018 Aug.
4
Chronic stress dampens excitatory synaptic gain in the paraventricular nucleus of the hypothalamus.慢性应激会抑制下丘脑室旁核中的兴奋性突触增益。
J Physiol. 2018 Sep;596(17):4157-4172. doi: 10.1113/JP275669. Epub 2018 Jul 22.
5
Endocannabinoids control vesicle release mode at midbrain periaqueductal grey inhibitory synapses.内源性大麻素在中脑导水管周围灰质抑制性突触处控制囊泡释放模式。
J Physiol. 2017 Jan 1;595(1):165-178. doi: 10.1113/JP272292. Epub 2016 Sep 15.
6
The ubiquitous nature of multivesicular release.多泡释放的普遍存在特性。
Trends Neurosci. 2015 Jul;38(7):428-38. doi: 10.1016/j.tins.2015.05.008. Epub 2015 Jun 19.
7
Stress-related synaptic plasticity in the hypothalamus.下丘脑与应激相关的突触可塑性。
Nat Rev Neurosci. 2015 Jul;16(7):377-88. doi: 10.1038/nrn3881.
8
Interplay between synchronization of multivesicular release and recruitment of additional release sites support short-term facilitation at hippocampal mossy fiber to CA3 pyramidal cells synapses.多泡释放同步化与额外释放位点募集之间的相互作用支持海马苔藓纤维至CA3锥体细胞突触处的短期易化。
J Neurosci. 2014 Aug 13;34(33):11032-47. doi: 10.1523/JNEUROSCI.0847-14.2014.
9
Short-term potentiation of GABAergic synaptic inputs to vasopressin and oxytocin neurones.向加压素和催产素神经元的GABA能突触输入的短期增强。
J Physiol. 2014 Oct 1;592(19):4221-33. doi: 10.1113/jphysiol.2014.277293. Epub 2014 Jul 25.
10
Role of intracellular calcium stores in hair-cell ribbon synapse.细胞内钙库在毛细胞突触中的作用。
Front Cell Neurosci. 2014 Jun 12;8:162. doi: 10.3389/fncel.2014.00162. eCollection 2014.
本文引用的文献
1
Norepinephrine triggers release of glial ATP to increase postsynaptic efficacy.去甲肾上腺素触发胶质细胞三磷酸腺苷的释放，以增强突触后效能。
Nat Neurosci. 2005 Aug;8(8):1078-86. doi: 10.1038/nn1498. Epub 2005 Jul 3.
2
Quantal components of the end-plate potential.终板电位的量子成分。
J Physiol. 1954 Jun 28;124(3):560-73. doi: 10.1113/jphysiol.1954.sp005129.
3
Priming of excitatory synapses by alpha1 adrenoceptor-mediated inhibition of group III metabotropic glutamate receptors.α1肾上腺素能受体介导的Ⅲ型代谢型谷氨酸受体抑制对兴奋性突触的启动作用。
J Neurosci. 2003 Jul 16;23(15):6223-31. doi: 10.1523/JNEUROSCI.23-15-06223.2003.
4
Presynaptic noradrenergic regulation of glutamate inputs to hypothalamic magnocellular neurones.去甲肾上腺素能对下丘脑大细胞神经元谷氨酸能输入的突触前调节。
J Neuroendocrinol. 2003 Aug;15(8):803-10. doi: 10.1046/j.1365-2826.2003.01063.x.
5
Nongenomic glucocorticoid inhibition via endocannabinoid release in the hypothalamus: a fast feedback mechanism.通过下丘脑内源性大麻素释放实现的非基因组糖皮质激素抑制：一种快速反馈机制。
J Neurosci. 2003 Jun 15;23(12):4850-7. doi: 10.1523/JNEUROSCI.23-12-04850.2003.
6
Modulation of presynaptic store calcium induces release of glutamate and postsynaptic firing.突触前储存钙的调节诱导谷氨酸释放和突触后放电。
Neuron. 2003 Jun 19;38(6):929-39. doi: 10.1016/s0896-6273(03)00322-2.
7
Independent sources of quantal variability at single glutamatergic synapses.单个谷氨酸能突触处量子变异性的独立来源。
J Neurosci. 2003 Apr 15;23(8):3186-95. doi: 10.1523/JNEUROSCI.23-08-03186.2003.
8
Quantal size and variation determined by vesicle size in normal and mutant Drosophila glutamatergic synapses.在正常和突变型果蝇谷氨酸能突触中，量子大小和变异由囊泡大小决定。
J Neurosci. 2002 Dec 1;22(23):10267-76. doi: 10.1523/JNEUROSCI.22-23-10267.2002.
9
Diversification of synaptic strength: presynaptic elements.突触强度的多样化：突触前成分
Nat Rev Neurosci. 2002 Jul;3(7):497-516. doi: 10.1038/nrn876.
10
Facilitation at single synapses probed with optical quantal analysis.用光量子分析探测单突触的易化作用。
Nat Neurosci. 2002 Jul;5(7):657-64. doi: 10.1038/nn867.
Zotero 插件