Flaherty Lorraine, Herron Bruce, Symula Derek
Genomics Institute, Wadsworth Center, Troy, New York 12180, USA.
Genome Res. 2005 Dec;15(12):1741-5. doi: 10.1101/gr.3841405.
Positional cloning of quantitative trait loci in rodents is a common approach to identify genes involved in complex phenotypes, including genes important to human disease. However, cloning the causative genes has proved to be more difficult than determining their positions. New tools such as genomic sequence, clone libraries, and new genomic-based methods offer new approaches to identify these genes. Here we review how these new tools and approaches will improve our ability to discover the genes important in complex traits.
在啮齿动物中进行数量性状基因座的定位克隆是鉴定参与复杂表型的基因（包括对人类疾病重要的基因）的常用方法。然而，事实证明克隆致病基因比确定它们的位置更加困难。诸如基因组序列、克隆文库和新的基于基因组的方法等新工具提供了鉴定这些基因的新途径。在此，我们综述这些新工具和方法将如何提高我们发现复杂性状中重要基因的能力。
