Bortiri Esteban, Jackson Dave, Hake Sarah
Plant Gene Expression Center, USDA-ARS, and Plant and Microbial Biology Department, University of California, Berkeley, 800 Buchanan Avenue, Albany, California 94710, USA.
Curr Opin Plant Biol. 2006 Apr;9(2):164-71. doi: 10.1016/j.pbi.2006.01.006. Epub 2006 Feb 2.
Positional cloning has been and remains a powerful method for gene identification in Arabidopsis. With the completion of the rice genome sequence, positional cloning in rice also took off, including the cloning of several quantitative trait loci. Positional cloning in cereals such as maize whose genomes are much larger than that of rice was considered near impossible because of the vast amounts of repetitive DNA. However, conservation of synteny across the cereal genomes, in combination with new maize resources, has now made positional cloning in maize feasible. In fact, a chromosomal walk is usually much faster than the more traditional method of gene isolation in maize by transposon tagging.
定位克隆一直是并仍然是拟南芥基因鉴定的一种强大方法。随着水稻基因组序列的完成，水稻中的定位克隆也开始兴起，包括几个数量性状位点的克隆。由于存在大量重复DNA，在基因组比水稻大得多的谷物如玉米中进行定位克隆曾被认为几乎是不可能的。然而，谷物基因组间的共线性保守性，再加上新的玉米资源，现在已使玉米中的定位克隆成为可行。事实上，染色体步移通常比通过转座子标签在玉米中进行基因分离的更传统方法要快得多。
