Suppr超能文献

德国小蠊的表达序列标签分析

Expressed sequence tags analysis of Blattella germanica.

作者信息

Chung Hyang Suk, Yu Tai Hyun, Kim Bong Jin, Kim Sun Mi, Kim Joo Yeong, Yu Hak Sun, Jeong Hae Jin, Ock Mee Sun

机构信息

Department of Otorhinolaryngology, Kosin University College of Medicine, Busan, Korea.

出版信息

Korean J Parasitol. 2005 Dec;43(4):149-56. doi: 10.3347/kjp.2005.43.4.149.

DOI:10.3347/kjp.2005.43.4.149
PMID:16340304
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2712019/
Abstract

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

摘要

从德国小蠊的一个cDNA文库中随机挑选出465个克隆进行部分测序，并使用BLAST进行搜索，以此作为分析其基因组转录序列的一种手段。在编辑和修剪载体及模糊序列后，从465个克隆中总共获得了363个表达序列标签（EST）。这些克隆中约42%（154/363）与其他数据库中登记的基因显示出显著的同源性。这些新的德国小蠊基因构成了广泛的转录本，分布在核糖体蛋白、能量代谢、过敏原、蛋白酶、蛋白酶抑制剂、酶、翻译、细胞信号通路以及功能未知的蛋白质中。有80个克隆在数据库搜索中匹配度不佳，这些代表了新的德国小蠊特异性EST。文中讨论了一些引起我们注意的基因。所获得的信息增进了我们对德国小蠊基因组的了解。

相似文献

1
Expressed sequence tags analysis of Blattella germanica.
Korean J Parasitol. 2005 Dec;43(4):149-56. doi: 10.3347/kjp.2005.43.4.149.
2
Vitellogenin of Blattella germanica (L.) (Dictyoptera, blattellidae): nucleotide sequence of the cDNA and analysis of the protein primary structure.
Arch Insect Biochem Physiol. 2000 Sep;45(1):1-11. doi: 10.1002/1520-6327(200009)45:1<1::AID-ARCH1>3.0.CO;2-O.
3
Identification and functional characterization of an ovarian aquaporin from the cockroach Blattella germanica L. (Dictyoptera, Blattellidae).
J Exp Biol. 2011 Nov 1;214(Pt 21):3630-8. doi: 10.1242/jeb.057406.
4
[R1 and R2 retrotransposons of German cockroach Blattella germanica: comparative analysis of 5' truncated copies integrated into genome].
Mol Biol (Mosk). 2007 Jul-Aug;41(4):608-15.
5
Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen.
Korean J Parasitol. 2005 Sep;43(3):101-10. doi: 10.3347/kjp.2005.43.3.101.
6
Anisakis simplex: analysis of expressed sequence tags (ESTs) of third-stage larva.
Exp Parasitol. 2007 Sep;117(1):51-6. doi: 10.1016/j.exppara.2007.03.009. Epub 2007 Mar 27.
7
Ovarian morphological features and proteome reveal fecundity fitness disadvantages in β-cypermethrin-resistant strains of Blattella germanica (L.) (Blattodea: Blattellidae).
Pestic Biochem Physiol. 2020 Nov;170:104682. doi: 10.1016/j.pestbp.2020.104682. Epub 2020 Aug 18.
8
Expression analysis of immune-relevant genes in the spleen of large yellow croaker (Pseudosciaena crocea) stimulated with poly I:C.
Fish Shellfish Immunol. 2006 Oct;21(4):414-30. doi: 10.1016/j.fsi.2006.01.006. Epub 2006 Apr 5.
9
Characteristics of the structure of minor variants of external transcribed spacers of ribosomal DNA of the cockroach Blattella germanica.
Dokl Biol Sci. 2005 Sep-Oct;404:348-51. doi: 10.1007/s10630-005-0132-8.
10
CYP9E2, CYP4C21 and related pseudogenes from German cockroaches, Blattella germanica: implications for molecular evolution, expression studies and nomenclature of P450s.
Gene. 2001 Jul 11;272(1-2):257-66. doi: 10.1016/s0378-1119(01)00529-7.

引用本文的文献

1
Cockroach allergen serine proteinases: Isolation, sequencing and signalling via proteinase-activated receptor-2.
Clin Exp Allergy. 2017 Jul;47(7):946-960. doi: 10.1111/cea.12921. Epub 2017 Apr 27.
2
De Novo Transcriptome Analysis and Detection of Antimicrobial Peptides of the American Cockroach Periplaneta americana (Linnaeus).
PLoS One. 2016 May 11;11(5):e0155304. doi: 10.1371/journal.pone.0155304. eCollection 2016.
3
Midgut Transcriptome of the Cockroach Periplaneta americana and Its Microbiota: Digestion, Detoxification and Oxidative Stress Response.
PLoS One. 2016 May 6;11(5):e0155254. doi: 10.1371/journal.pone.0155254. eCollection 2016.
4
De novo Assembly and Characterization of the Testis Transcriptome and Development of EST-SSR Markers in the Cockroach Periplaneta americana.
Sci Rep. 2015 Jun 5;5:11144. doi: 10.1038/srep11144.
5
Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach.
Allergy Asthma Immunol Res. 2015 May;7(3):283-9. doi: 10.4168/aair.2015.7.3.283. Epub 2014 Dec 18.
6
De novo transcriptome of the Hemimetabolous German cockroach (Blattella germanica).
PLoS One. 2014 Sep 29;9(9):e106932. doi: 10.1371/journal.pone.0106932. eCollection 2014.
7
Identification of differentially expressed genes in American cockroach ovaries and testes by suppression subtractive hybridization and the prediction of its miRNAs.
Mol Genet Genomics. 2013 Nov;288(11):627-38. doi: 10.1007/s00438-013-0777-1. Epub 2013 Aug 31.

本文引用的文献

1
Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen.
Korean J Parasitol. 2005 Sep;43(3):101-10. doi: 10.3347/kjp.2005.43.3.101.
2
The complete nucleotide sequence and gene organization of the mitochondrial genome of the oriental mole cricket, Gryllotalpa orientalis (Orthoptera: Gryllotalpidae).
Gene. 2005 Jul 4;353(2):155-68. doi: 10.1016/j.gene.2005.04.019.
3
The mitochondrial genome of the firefly, Pyrocoelia rufa: complete DNA sequence, genome organization, and phylogenetic analysis with other insects.
Mol Phylogenet Evol. 2004 Sep;32(3):978-85. doi: 10.1016/j.ympev.2004.03.009.
4
Genome update: Length distributions of sequenced prokaryotic genomes.
Microbiology (Reading). 2004 Mar;150(Pt 3):513-516. doi: 10.1099/mic.0.27032-0.
5
Annotation pattern of ESTs from Spodoptera frugiperda Sf9 cells and analysis of the ribosomal protein genes reveal insect-specific features and unexpectedly low codon usage bias.
Bioinformatics. 2003 Dec 12;19(18):2343-50. doi: 10.1093/bioinformatics/btg324.
6
Overexpression of the cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella germanica (L.).
Insect Biochem Mol Biol. 2003 Oct;33(10):1043-8. doi: 10.1016/s0965-1748(03)00120-6.
7
Drosophila S3 ribosomal protein accelerates repair of 8-oxoguanine performed by human and mouse cell extracts.
Environ Mol Mutagen. 2003;42(1):50-8. doi: 10.1002/em.10166.
8
PlasmoDB: the Plasmodium genome resource. A database integrating experimental and computational data.
Nucleic Acids Res. 2003 Jan 1;31(1):212-5. doi: 10.1093/nar/gkg081.
9
Longevity determination genes in Drosophila melanogaster.
Mech Ageing Dev. 2002 Nov;123(12):1531-41. doi: 10.1016/s0047-6374(02)00089-1.
10
Structure and function of the eukaryotic ribosome: the next frontier.
Cell. 2002 Apr 19;109(2):153-6. doi: 10.1016/s0092-8674(02)00725-0.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验