Jacoby A F, Young R H, Colvin R B, Flotte T J, Preffer F, Scully R E, Swymer C M, Bell D A
Department of Pathology, Harvard Medical School, Boston, Massachusetts.
Gynecol Oncol. 1992 Jul;46(1):97-103. doi: 10.1016/0090-8258(92)90203-u.
Paraffin-embedded tissue from 38 patients with early- and advanced-stage juvenile granulosa cell tumor (JGCT) of the ovary was analyzed by flow cytometry to investigate whether the DNA content is related to the histologic features, stage, or clinical outcome. Thirty-three cases were suitable for analysis: twenty-seven Stage Ia, two Stage Ic, and four Stage III. Eighteen (55%) tumors were DNA diploid and fifteen (45%) tumors were DNA aneuploid with a mean S-phase fraction (SPF) of 13.6% and a range of DNA indices from 1.0 to 2.2. Neither the DNA ploidy nor the SPF was associated with the stage of the disease. An analysis of the relation between DNA content and histopathologic features revealed that aneuploidy was associated with high mitotic rates and to a lesser extent with high-grade nuclear atypia. DNA aneuploidy was not associated with aggressive behavior of Stage Ia JGCTs. However, among the four patients with Stage III tumors, the two with diploid, low-SPF tumors were alive and well, whereas the two with aneuploid, high-SPF tumors developed recurrences or died. These data suggest that further studies on the prognostic significance of flow cytometric analysis of DNA content in advanced-stage JGCTs are warranted.
对38例卵巢早、晚期幼年型颗粒细胞瘤（JGCT）患者的石蜡包埋组织进行流式细胞术分析，以研究DNA含量是否与组织学特征、分期或临床结局相关。33例适合分析：27例为Ⅰa期，2例为Ⅰc期，4例为Ⅲ期。18例（55%）肿瘤为DNA二倍体，15例（45%）肿瘤为DNA非整倍体，平均S期分数（SPF）为13.6%，DNA指数范围为1.0至2.2。DNA倍体和SPF均与疾病分期无关。对DNA含量与组织病理学特征之间关系的分析显示，非整倍体与高有丝分裂率相关，在较小程度上与高级别核异型性相关。DNA非整倍体与Ⅰa期JGCT的侵袭性行为无关。然而，在4例Ⅲ期肿瘤患者中，2例二倍体、低SPF肿瘤患者存活且状况良好，而2例非整倍体、高SPF肿瘤患者出现复发或死亡。这些数据表明，有必要对晚期JGCT中DNA含量的流式细胞术分析的预后意义进行进一步研究。
