使用胶束介质和荧光胺作为标记试剂，通过荧光法测定鱼类中的组胺。

Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent.

作者信息

Adamou Rabani, Coly Atanasse, Douabalé Stephy Edgar, Saleck Med Lemine Ould Cheikh Ould, Gaye-Seye Mame Diabou, Tine Alphonse

机构信息

Faculté des Sciences et Techniques, Département de Chimie, Laboratoire de Photochimie et d'Analyse, Université Cheikh Anta Diop, Dakar, Sénégal.

出版信息

J Fluoresc. 2005 Sep;15(5):679-88. doi: 10.1007/s10895-005-2975-7.

DOI:10.1007/s10895-005-2975-7

PMID:16341785

Abstract

An analytical method based on the use of fluorescamine to produce a fluorescent derivative with histamine and combined with micellar-enhanced fluorescence detection of the formed complex is developed for the sensitive and rapid determination of histamine in fishes. The fluorescence properties of the obtained complex in water and micellar solutions of sodium dodecyl sulfate (SDS), cetyltrimethylammonium chloride (CTAC) and brij-700 are reported. Physicochemical variables influencing the sensitivity of the method (pH, micellar, fluorescamine and NaCl relative concentrations) have been optimized. The stability of the formed complex, as shown by kinetic study, depends on the pH of the solution. Linear calibration curves allowing an effective histamine determination were established with large linear dynamic range (LDR), and low limits of detection (LOD) between 0.5 and 33 ng mL-1, according to the solvent. Application to the analysis of fish samples (sardines) yielded satisfactory results. The method seems to be suitable for environmental fish quality control.

摘要

开发了一种基于使用荧光胺与组胺生成荧光衍生物，并结合对形成的络合物进行胶束增强荧光检测的分析方法，用于灵敏、快速地测定鱼类中的组胺。报道了所获得的络合物在水以及十二烷基硫酸钠（SDS）、十六烷基三甲基氯化铵（CTAC）和Brij - 700胶束溶液中的荧光性质。对影响该方法灵敏度的物理化学变量（pH值、胶束、荧光胺和氯化钠的相对浓度）进行了优化。动力学研究表明，形成的络合物的稳定性取决于溶液的pH值。根据溶剂不同，建立了具有大线性动态范围（LDR）和0.5至33 ng mL-1的低检测限（LOD）的线性校准曲线，可有效测定组胺。将该方法应用于鱼样（沙丁鱼）分析，结果令人满意。该方法似乎适用于环境鱼类质量控制。