绿色荧光蛋白转基因植物的激光诱导荧光成像与光谱分析

Laser-induced fluorescence imaging and spectroscopy of GFP transgenic plants.

作者信息

Stewart C Neal, Millwood Reginald J, Halfhill Matthew D, Ayalew Mentewab, Cardoza Vinitha, Kooshki Mitra, Capelle Gene A, Kyle Kevin R, Piaseki David, McCrum Gregory, Di Benedetto John

机构信息

Department of Plant Sciences, University of Tennessee, Knoxville, Tennesssee 37996, USA.

出版信息

J Fluoresc. 2005 Sep;15(5):697-705. doi: 10.1007/s10895-005-2977-5.

DOI:10.1007/s10895-005-2977-5

PMID:16341787

Abstract

Green fluorescent protein (GFP) and other fluorescent protein bioreporters can be used to monitor transgenes in plants. GFP is a valuable marker for transgene presence and expression, but remote sensing instrumentation for stand-off detection has lagged behind fluorescent protein marker biotechnology. However, both biology and photonics are needed for the monitoring technology to be fully realized. In this paper, we describe laser-induced fluorescence imaging and laser-induced fluorescence spectroscopy of GFP-transgenic plants in ambient light towards the application of remote sensing of transgenic plants producing GFP.

摘要

绿色荧光蛋白（GFP）和其他荧光蛋白生物报告基因可用于监测植物中的转基因。GFP是转基因存在和表达的重要标记，但用于远距离检测的遥感仪器落后于荧光蛋白标记生物技术。然而，监测技术要完全实现需要生物学和光子学两者的结合。在本文中，我们描述了在自然光下对绿色荧光蛋白转基因植物进行激光诱导荧光成像和激光诱导荧光光谱分析，以应用于对产生绿色荧光蛋白的转基因植物进行遥感。