Wu W K, Mak C H, Ko R C
Department of Zoology, The University of Hong Kong, Pokfulam, Hong Kong, SAR, China.
Parasitol Res. 2006 Mar;98(4):281-7. doi: 10.1007/s00436-005-0056-0. Epub 2005 Dec 10.
Copper/zinc (Cu/Zn) superoxide dismutase (SOD) activity was identified for the first time in both crude somatic extracts (CE) and excretory/secretory (E/S) products of Trichinella pseudospiralis. It was the dominant SOD in infective-stage larvae. Native polyacrylamide gel electrophoresis of CE and E/S products yielded a prominent band, which was cyanide-sensitive and was partly inhibited by hydrogen peroxide in SOD assay. Cytosolic Cu/Zn SOD was cloned. The 471-bp full-length cDNA sequence contained an open reading frame of 157 amino acids. The gene contained three introns. Quantitative reverse transcription-polymerase chain reaction indicated that the expression of cytosolic Cu/Zn SOD was substantially higher in infective-stage larvae than in adult worms. Cluster analysis showed that the sequence of the Cu/Zn SOD of T. pseudospiralis, an adenophorean nematode, is related to those of Brugia pahangi, Acanthocheilonema viteae, Onchocerca volvulus, and Haemonchus contortus (all belonging to the sercenentean group).
首次在旋毛虫的粗体壁提取物（CE）和排泄/分泌（E/S）产物中鉴定出铜/锌（Cu/Zn）超氧化物歧化酶（SOD）活性。它是感染期幼虫中的主要SOD。对CE和E/S产物进行的非变性聚丙烯酰胺凝胶电泳产生了一条明显的条带，该条带对氰化物敏感，并且在SOD测定中部分受过氧化氢抑制。克隆了胞质Cu/Zn SOD。471 bp的全长cDNA序列包含一个157个氨基酸的开放阅读框。该基因包含三个内含子。定量逆转录-聚合酶链反应表明，胞质Cu/Zn SOD在感染期幼虫中的表达明显高于成虫。聚类分析表明，腺口线虫旋毛虫的Cu/Zn SOD序列与马来布鲁线虫、旋盘尾丝虫、盘尾丝虫和捻转血矛线虫（均属于丝虫类） 的序列相关。
