在体外，褪黑素处理可降低与委内瑞拉马脑炎病毒一起培养的小鼠脾细胞中的一氧化氮水平。

In vitro, melatonin treatment decreases nitric oxide levels in murine splenocytes cultured with the venezuelan equine encephalomyelitis virus.

作者信息

Valero Nereida, Meleán Eddy, Bonilla Ernesto, Arias Julia, Espina Luz Marina, Chacin-Bonilla Leonor, Larreal Yraima, Maldonado Merybell, Añez Florencio

机构信息

Secció n de VirologiaInstituto de Investigaciones Clínicas Dr. Américo Negrette, Facultad de Medicina, Universidad del Zulia, Apartado Postal 23, Maracaibo, Estado Zulia, Venezuela.

出版信息

Neurochem Res. 2005 Nov;30(11):1439-42. doi: 10.1007/s11064-005-8634-1.

DOI:10.1007/s11064-005-8634-1

PMID:16341941

Abstract

The purpose of this work was to determine the effect of melatonin on the nitric oxide levels in murine splenocytes cultured with the Venezuelan equine encephalomyelitis virus. After incubation, nitric oxide levels were measured by the diazotization assay. Those cultures with the Venezuelan equine encephalomyelitis virus increased nitric oxide levels. Splenocytes infected and treated with 100 and 150 microg/ml of melatonin, decreased significantly the nitric oxide levels when compared to infected and non-treated splenocytes. These findings show that splenocytes infected with the Venezuelan equine encephalomyelitis virus generate important amounts of nitric oxide and suggest that melatonin protects the mice infected with the Venezuelan equine encephalomyelitis virus by a mechanism involving the decreasing of nitric oxide concentrations in tissue.

摘要

本研究的目的是确定褪黑素对感染委内瑞拉马脑炎病毒的小鼠脾细胞中一氧化氮水平的影响。孵育后，通过重氮化测定法测量一氧化氮水平。感染委内瑞拉马脑炎病毒的培养物中一氧化氮水平升高。与未处理的感染脾细胞相比，用100和150微克/毫升褪黑素感染并处理的脾细胞显著降低了一氧化氮水平。这些发现表明，感染委内瑞拉马脑炎病毒的脾细胞会产生大量一氧化氮，并提示褪黑素通过降低组织中一氧化氮浓度的机制保护感染委内瑞拉马脑炎病毒的小鼠。